An Efficient Method for Extraction, Separation and Purification of Naphthoquinone Pigments from Lithospermum erythrorhizon Sieb. et Zucc. by SFE and HSCCC

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作者: By:Feng, L [ 2 ] Ji, HW [ 2 ] Gu, HX [ 3 ] Wang, DC [ 2 ] Cui, JC [ 4 ] Liu, RM [ 4 ] Zhai, J [ 1 ]

作者单位: Cent Hosp Taian, Dept Pharm, Tai An 271016, Shandong, Peoples R China Liaocheng Univ, Coll Chem & Chem Engn, Liaocheng 252059, Peoples R China Organization-Enhanced Name(s) Liaocheng University Taishan Med Univ, Dept Biosci, Tai An 271016, Shandong, Peoples R China Organization-Enhanced Name(s) Taishan Medical University Taishan Med Univ, Dept Basic Med, Tai An 271000, Shandong, Peoples R China Organization-Enhanced Name(s) Taishan Medical University

关键词: Column liquid chromatography Supercritical fluid chromatography Counter-current chromatography Acetylshikonin and isobutyrylshikonin Lithospermum erythrorhizon Sieb. et Zucc

摘要: Supercritical fluid extraction was used to extract naphthoquinone pigments from Lithospermum erythrorhizon Sieb. et Zucc. The crude extracts were separated and purified by high-speed counter-current chromatography with light petroleum–ethyl acetate–methanol–water (5:5:8:2, v/v) as the two-phase solvent system. Three kinds of naphthoquinone pigments including 17.6 mg of β-hydroxyisovalerylshikonin (I), 17.6 mg of acetylshikonin (II), and 19.7 mg of isobutyrylshikonin (III) were obtained from 150 ...更多; Supercritical fluid extraction was used to extract naphthoquinone pigments from Lithospermum erythrorhizon Sieb. et Zucc. The crude extracts were separated and purified by high-speed counter-current chromatography with light petroleum–ethyl acetate–methanol–water (5:5:8:2, v/v) as the two-phase solvent system. Three kinds of naphthoquinone pigments including 17.6 mg of β-hydroxyisovalerylshikonin (I), 17.6 mg of acetylshikonin (II), and 19.7 mg of isobutyrylshikonin (III) were obtained from 150 mg crude sample. The purity of these compounds was 96.7, 99.3 and 95.5%, respectively, as determined by liquid chromatograph. Their structures were identified by 1 H NMR and 13 C NMR.收起

学科: 【SCI学科】BIOCHEMICAL RESEARCH METHODS;CHEMISTRY, ANALYTICAL 【ESI学科】CHEMISTRY

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