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作者

By:Feng, L [ 2 ] Ji, HW [ 2 ] Gu, HX [ 3 ] Wang, DC [ 2 ] Cui, JC [ 4 ] Liu, RM [ 4 ] Zhai, J [ 1 ]

作者单位

Cent Hosp Taian, Dept Pharm, Tai An 271016, Shandong, Peoples R China Liaocheng Univ, Coll Chem & Chem Engn, Liaocheng 252059, Peoples R China Organization-Enhanced Name(s) Liaocheng University Taishan Med Univ, Dept Biosci, Tai An 271016, Shandong, Peoples R China Organization-Enhanced Name(s) Taishan Medical University Taishan Med Univ, Dept Basic Med, Tai An 271000, Shandong, Peoples R China Organization-Enhanced Name(s) Taishan Medical University

刊名

Chromatographia

年份

2009

卷号

Vol.70 No.7-8

页码

1197-1200

ISSN

0009-5893

关键词

Column liquid chromatography Supercritical fluid chromatography Counter-current chromatography Acetylshikonin and isobutyrylshikonin Lithospermum erythrorhizon Sieb. et Zucc

分类号

543.8

摘要

Supercritical fluid extraction was used to extract naphthoquinone pigments from Lithospermum erythrorhizon Sieb. et Zucc. The crude extracts were separated and purified by high-speed counter-current chromatography with light petroleum–ethyl acetate–methanol–water (5:5:8:2, v/v) as the two-phase solvent system. Three kinds of naphthoquinone pigments including 17.6 mg of β-hydroxyisovalerylshikonin (I), 17.6 mg of acetylshikonin (II), and 19.7 mg of isobutyrylshikonin (III) were obtained from 150 ...更多

Supercritical fluid extraction was used to extract naphthoquinone pigments from Lithospermum erythrorhizon Sieb. et Zucc. The crude extracts were separated and purified by high-speed counter-current chromatography with light petroleum–ethyl acetate–methanol–water (5:5:8:2, v/v) as the two-phase solvent system. Three kinds of naphthoquinone pigments including 17.6 mg of β-hydroxyisovalerylshikonin (I), 17.6 mg of acetylshikonin (II), and 19.7 mg of isobutyrylshikonin (III) were obtained from 150 mg crude sample. The purity of these compounds was 96.7, 99.3 and 95.5%, respectively, as determined by liquid chromatograph. Their structures were identified by 1 H NMR and 13 C NMR.收起

学科

【SCI学科】BIOCHEMICAL RESEARCH METHODS;CHEMISTRY, ANALYTICAL 【ESI学科】CHEMISTRY

影响因子

2016年:1.402;2015年:1.332;2014年:1.411;2013年:1.37;2012年:1.437

文献类型

期刊