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作者

Cao Shuang Wang Guohua Wang Jia Li Cheng Zhang Le

作者单位

Department of Electroneurophysiology, Qilu Children's Hospital of Shandong University, Jinan, 250022, Shandong, China. Department of Gynecology, Affiliated Hospital of Taishan Medical University, Taian, 271000, Shandong, China. School of Public Health, Taishan Medical University, Taian, 271016, Shandong, China.

刊名

Immunology and cell biology

年份

2018

ISSN

1440-1711

关键词

JAK2 Hepatocellular carcinoma hsa_circ_101280 miR-375

分类号

616.97, 570

摘要

In this study, we sought to predict the effects of a certain circular RNA , hsa_circ_101280 , on hepatocellular carcinoma cells and to determine the potential mechanism. After screening differentially expressed circRNAs in HCC tissues through GEO data analysis, hsa_circ_101280 was found to be highly expressed, and its high expression was verified in HCC cell lines with qRT-PCR along with the low expression of its downstream miRNA miR-375. Colony formation and flow cytometry assays showed that b...更多

In this study, we sought to predict the effects of a certain circular RNA , hsa_circ_101280 , on hepatocellular carcinoma cells and to determine the potential mechanism. After screening differentially expressed circRNAs in HCC tissues through GEO data analysis, hsa_circ_101280 was found to be highly expressed, and its high expression was verified in HCC cell lines with qRT-PCR along with the low expression of its downstream miRNA miR-375. Colony formation and flow cytometry assays showed that both hsa_circ_101280 silencing and miR-375 overexpression restrained proliferation and promoted apoptosis in HCC cells. JAK2 was identified as a downstream mRNA target of miR-375 by RNA pull-down and dual-luciferase reporter gene assays, its expression in HCC cell lines were positively regulated by hsa_circ_101280 and negatively by miR-375 expression. Furthermore, silencing of has_circ_101280 significantly inhibited the growth of HCC xenografts in nude mice, with the down-regulated expression of JAK2. Overall, both the in vitro and in vivo studies revealed that hsa_circ_101280 largely facilitated the tumorigenesis of HCC, characterized by the promoted proliferation and suppressed apoptosis of HCC cells, by sponging miR-375 and up-regulating JAK2. This article is protected by copyright. All rights reserved.收起

学科

【SCI学科】IMMUNOLOGY;CELL BIOLOGY 【ESI学科】IMMUNOLOGY

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