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作者

Cao, Shuang Wang, Guohua Wang, Jia Li, Cheng Zhang, Le

作者单位

Department of Electroneurophysiology, Qilu Children's Hospital of Shandong University, Jinan Shandong, 250022, China Department of Gynecology, Affiliated Hospital of Taishan Medical University, Taian Shandong, 271000, China School of Public Health, Taishan Medical University, Taian Shandong, 271016, China

刊名

Immunology & Cell Biology

年份

2019

卷号

Vol.97 No.2

页码

218-228

ISSN

0818-9641

关键词

Hepatocellular carcinoma hsacirc101280 JAK2 miR‐375

摘要

In this study, we sought to predict the effects of a certain circular RNA , hsa_circ_101280 , on hepatocellular carcinoma cells and to determine the potential mechanism. After screening differentially expressed circRNAs in HCC tissues through Gene Expression Omnibus data analysis, hsa_circ_101280 was found to be highly expressed, and its high expression was verified in HCC cell lines with qRT‐PCR along with the low expression of its downstream miRNA miR‐375. Colony formation and flow cytometry ...更多

In this study, we sought to predict the effects of a certain circular RNA , hsa_circ_101280 , on hepatocellular carcinoma cells and to determine the potential mechanism. After screening differentially expressed circRNAs in HCC tissues through Gene Expression Omnibus data analysis, hsa_circ_101280 was found to be highly expressed, and its high expression was verified in HCC cell lines with qRT‐PCR along with the low expression of its downstream miRNA miR‐375. Colony formation and flow cytometry assays showed that both hsa_circ_101280 silencing and miR‐375 overexpression restrained proliferation and promoted apoptosis in HCC cells. JAK2 was identified as a downstream mRNA target of miR‐375 by RNA pull‐down and dual‐luciferase reporter gene assays, its expression in HCC cell lines were positively regulated by hsa_circ_101280 and negatively by miR‐375 expression. Furthermore, the silencing of hsa_circ_101280 significantly inhibited the growth of HCC xenografts in nude mice, with the downregulated expression of JAK2. Overall, both the in vitro and in vivo studies revealed that hsa_circ_101280 largely facilitated the tumorigenesis of HCC, characterized by the promoted proliferation and suppressed apoptosis of HCC cells, by sponging miR‐375 and upregulating JAK2. hsa_circ_101280, miR‐375 and JAK2 were differentially expressed in hepatocellular carcinoma cells, and hsa_circ_101280 regulated cell proliferation and apoptosis by inhibiting/promoting the expression of miR‐375/JAK2 in HCC cells.收起

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