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作者

Wang Xianjue Wei Ping Yang Ling Liu Fangyuan Tong Xin Yang Xiaoyu Su Liya

作者单位

¹Clinical Medical Research Center of the Affiliated Hospital；²Inner Mongolia Medical University；³Inner Mongolia Key Laboratory of Medical Cell Biology；⁴Hohhot；⁵Inner Mongolia 010050；⁶China；⁷Department of Medical Immunology；⁸Basic Medical College；⁹Shandong First Medical University and Shandong Academy of Medical Sciences；¹⁰Taian；¹¹Shandong 271000

刊名

中华医学杂志英文版

年份

2022

卷号

第135卷

期号

第17期

页码

2089-2097

ISSN

0366-6999

关键词

Epithelial-mesenchymal transition Hepatocellular carcinoma miR-20a-5p RUNX3

分类号

R735.7

摘要

Background: MicroRNA-20a is dysregulated in many types of malignancies, including human hepatocellular carcinoma , but its expression level and functional significance in HCC are still disputed. We aimed to study the role of miR-20a-5p in HCC and its downstream molecular mechanisms.Methods: We used real-time polymerase chain reaction to detect the expression of miR-20a-5p and runt-related transcription factor 3 in HCC and paraneoplastic tissue, transfected Huh7 and highly metastatic human hepa...更多

Background: MicroRNA-20a is dysregulated in many types of malignancies, including human hepatocellular carcinoma , but its expression level and functional significance in HCC are still disputed. We aimed to study the role of miR-20a-5p in HCC and its downstream molecular mechanisms.Methods: We used real-time polymerase chain reaction to detect the expression of miR-20a-5p and runt-related transcription factor 3 in HCC and paraneoplastic tissue, transfected Huh7 and highly metastatic human hepatocellular carcinoma cells. A live cell workstation was used to observe the proliferation and migration of transfected cells. The invasiveness of transfected cells was verified by Transwell assay. Cell apoptosis was detected by flow cytometry. The expression levels of proteins after transfection were measured using simple western immunoblot measurements. Gene expression profiles between HCC and normal samples were obtained from The Cancer Genome Atlas. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment results were processed by the database for annotation, visualization and integrated discovery. Potential target genes of miR-20a-5p were predicted to further investigate how miR-20a-5p regulates epithelial-mesenchymal transition in HCC.Results: MiR-20a-5p was significantly highly expressed in HCC tissues, and overexpression of miR-20a-5p significantly promoted HCC cell proliferation, migration, and invasion and inhibited apoptosisin vitro. The protein expression of E-cadherin was decreased and that of vimentin was increased after overexpression of miR-20a-5p in HCC cells. We discovered the intersection of genes from miRDB, miR TarBase, and TargetScan, obtained 397 target genes and finally focused onRUNX3. RUNX3 was not only reduced in HCC specimens but also drastically reduced in HCC cells overexpressing miR-20a-5p.RUNX3 expression decreased with elevated miR-20a-5p, which activated downstream EMT signaling and promoted cell proliferation, migration, and invasion.Conclusions: SinceRUNX3 is involved in EMT in HCC, as proven by previous research, our findings provide further evidence for a novel regulatory pathway comprising the miR-20a/RUNX3/EMT axis that upregulates EMT signaling and enhances the migration of HCC cells.收起

学科

【教育部学科】医学

文献类型

期刊

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