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作者

Su, Y.-P.Email Author, Wang, H., Ge, L., Liu, L.-W., Wei, L.-H. View Correspondence

作者单位

Department of Histology and Embryology, Taishan Medical College, Shandong Taian 271000, China

刊名

Acta Anatomica Sinica

年份

2011

卷号

Vol.42 No.2

页码

180-184

ISSN

0529-1356

关键词

EMTREE drug terms sodium selenite telomerase reverse transcriptase EMTREE medical terms article cancer growth cancer inhibition controlled study drug activity human human cell immunohistochemistry inhibition kinetics protein blood level protein expression reverse transcription polymerase chain reaction stomach cancer

摘要

Objective: To investigate effects of sodium selenite on human gastric cancer SGC-7901 cell growth and hTERT expression and to explore the mechanism. Methods: SGC-7901 cells were treated with different concentrations of sodium selenite for 24 hours, 48 hours, 72 hours and 96 hours. Growth inhibition of SGC-7901 cells was detected by assay of MTT. Immunohistochemical SABC method and RT-PCR assay were used to study the changes of hTERT proteins and hTERT mRNA in SGC-7901 cells. Results: Sodium sel...更多

Objective: To investigate effects of sodium selenite on human gastric cancer SGC-7901 cell growth and hTERT expression and to explore the mechanism. Methods: SGC-7901 cells were treated with different concentrations of sodium selenite for 24 hours, 48 hours, 72 hours and 96 hours. Growth inhibition of SGC-7901 cells was detected by assay of MTT. Immunohistochemical SABC method and RT-PCR assay were used to study the changes of hTERT proteins and hTERT mRNA in SGC-7901 cells. Results: Sodium selenite significantly suppressed the growth of SGC-7901 cells. The cell growth rate reduced in a dose-and time-dependent manner. The same changes had occurred for the hTERT protein level in SGC-7901 cells. The averae absorbance of hTERT protein in SGC-7901 cells was gradually lower along time, unto 96 hour it was significantly decreased in 0. 5μmol/ L sodium selenite group or 2. 5μmol/L sodium selenite group than that in blank control group . It was significantly decreased in 5μmol/L, 8μmol/L sodium selenite group than in blank control group all time . RT-PCR results demonstrated there was no obvious alteration of hTERT mRNA levels after 0.5μmol/L sodium selenite treatment. hTERT mRNA levels were gradually reduced along time in 2. 5μmol/L sodium selenite group, there was significantly different after 48 hours treatment in 2. 5μmol/L sodium selenite group than in blank control group. hTERT mRNA levels were significantly decreased all time in 5μmol/L, 8μmol/L sodium selenite group than in blank control group , unto 72 hours it was significantly decreased than others lower sodium selenite groups . Conclusion: Sodium selenite suppressed the SGC-7901 cell growth, and down-regulated the hTERT expression.收起

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