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作者

Fengchun Zhao Yuan Tian Qiang Shen Ruxia Liu Ruirui Shi Huimin Wang Zhengyou Yang

作者单位

College of Life Science, Taishan Medical University, Taian 271016, China a Department of Microbiology, College of Life Science, Key Laboratory for Agriculture Microbiology, Shandong Agricultural University, Taian 271018, China

刊名

Talanta

年份

2019

卷号

Vol.195

页码

55-61

ISSN

0039-9140

关键词

Aflatoxin Mimotope Nanobody Phage-display Magnetic beads-based ELISA

分类号

543

摘要

Mimotopes could replace mycotoxins and their conjugates to develop immunoassay methods. The mimotopes obtained by phage display technology were mainly using monoclonal antibodies or polyclonal antibodies as targets. However, the mimotope of recombinant antibody has not been selected and applied to immunoassay for mycotoxin. The purpose of this study was to prove that an immunoassay for mycotoxin could be developed based on both recombinant antibody and its mimotope. Using aflatoxin B1 as a mode...更多

Mimotopes could replace mycotoxins and their conjugates to develop immunoassay methods. The mimotopes obtained by phage display technology were mainly using monoclonal antibodies or polyclonal antibodies as targets. However, the mimotope of recombinant antibody has not been selected and applied to immunoassay for mycotoxin. The purpose of this study was to prove that an immunoassay for mycotoxin could be developed based on both recombinant antibody and its mimotope. Using aflatoxin B1 as a model system, mimotopes of an aflatoxin nanobody Nb28 were screened by phage display. A rapid magnetic beads-based directed competitive ELISA was developed utilizing Nb28 and its mimotope ME17. The 50% inhibitory concentration and the detection limit of the MB-dcELISA were 0.75 and 0.13 ng/mL, respectively, with a linear range of 0.24–2.21 ng/mL. Further validation study indicated good recovery with low coefficient of variable in spiked corn, rice, peanut, feedstuff, corn germ oil and peanut oil samples. The developed immunoassay based on nanobody and mimotope provides a new strategy for the monitoring of AFB 1 and other toxic small molecular weight compounds.收起

学科

【SCI学科】CHEMISTRY, ANALYTICAL 【ESI学科】CHEMISTRY

影响因子

2016年:4.162;2015年:4.035;2014年:3.545;2013年:3.511;2012年:3.498

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