Department of Neurosurgery, Shandong University Qilu Hospital, Qingdao, Shandong, ChinaDepartment of Neurosurgery, Affiliated Hospital of Taishan Medical University, Tai An, Shandong, China
Background: This study aimed to analyze the relationship between molecular pathologic expression of GFAP and Ki-67 and fluorescence levels, and to provide molecular pathological basis for the removal of malignant gliomas by Fluorescein Sodium navigation under the YELLOW 560 nm surgical microscope filter. Methods: A retrospective analysis of clinical data of 18 MG cases confirmed by the postoperative pathology was performed. All cases were resected by FLS guiding under the YELLOW 560 nm filter....更多
Background: This study aimed to analyze the relationship between molecular pathologic expression of GFAP and Ki-67 and fluorescence levels, and to provide molecular pathological basis for the removal of malignant gliomas by Fluorescein Sodium navigation under the YELLOW 560 nm surgical microscope filter. Methods: A retrospective analysis of clinical data of 18 MG cases confirmed by the postoperative pathology was performed. All cases were resected by FLS guiding under the YELLOW 560 nm filter. Hematoxylin-eosin staining, molecular pathology markers GFAP, and Ki-67 immunohistochemical staining of the specimens were performed. The relationship between fluorescence staining levels and GFAP positive rate, Ki-67 proliferation index, and WHO grades was studied. Results: There were 69 pathological specimens with fluorescence levels of 'bright' fluorescence , 'low' fluorescence , and 'no' fluorescence . Immunohistochemical staining showed GFAP-positive expression in both tumor cells and normal glial cells. The staining levels of the specimens in the fluorescence regions were higher than that in the non-fluorescence regions. GFAP expression was positive in 61 specimens and negative in 8 specimens. Comparison of Ki-67 proliferation index using chi-square test showed different fluorescence levels had different Ki-67 proliferation indexes . With high proliferation index of specimens, fluorescence level was brighter. WHO grade had no correlation with fluorescence levels . Conclusion: FLS-guided resection of MG is safe and effective. In the boundary area of MG, fluorescence levels and Ki-67 proliferation index showed correlation. FLS-guided resection achieved the function of 'reducing tumor cell,' thus reducing the proliferation index in the lesion area.收起
发文期刊《Molecular pathological expression in malignant gliomas resected by fluorescein sodium-guiding under the YELLOW 560 nm surgical microscope filter.》历年引证文献趋势图
