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作者

Zhang, Ningning Shang, Zhende Wang, Zhigang Meng, Xianbing Li, Zheng Tian, Hailong Huang, Dezhang Yin, Xin Zheng, Bin Zhang, Xinhua

作者单位

Department of Neurosurgery, Shandong University Qilu Hospital, Qingdao, Shandong, China Department of Neurosurgery, Affiliated Hospital of Taishan Medical University, Tai An, Shandong, China

刊名

World Journal of Surgical Oncology

年份

2018

卷号

Vol.16 No.1

ISSN

1477-7819

关键词

MOLECULAR pathology *PATHOLOGICAL physiology *ASTROCYTOMAS *CHEMOTHERAPY (Cancer) *MICRORNA

分类号

616.992, 617

摘要

Background: This study aimed to analyze the relationship between molecular pathologic expression of GFAP and Ki-67 and fluorescence levels, and to provide molecular pathological basis for the removal of malignant gliomas by Fluorescein Sodium navigation under the YELLOW 560 nm surgical microscope filter. Methods: A retrospective analysis of clinical data of 18 MG cases confirmed by the postoperative pathology was performed. All cases were resected by FLS guiding under the YELLOW 560 nm filter....更多

Background: This study aimed to analyze the relationship between molecular pathologic expression of GFAP and Ki-67 and fluorescence levels, and to provide molecular pathological basis for the removal of malignant gliomas by Fluorescein Sodium navigation under the YELLOW 560 nm surgical microscope filter. Methods: A retrospective analysis of clinical data of 18 MG cases confirmed by the postoperative pathology was performed. All cases were resected by FLS guiding under the YELLOW 560 nm filter. Hematoxylin-eosin staining, molecular pathology markers GFAP, and Ki-67 immunohistochemical staining of the specimens were performed. The relationship between fluorescence staining levels and GFAP positive rate, Ki-67 proliferation index, and WHO grades was studied. Results: There were 69 pathological specimens with fluorescence levels of 'bright' fluorescence , 'low' fluorescence , and 'no' fluorescence . Immunohistochemical staining showed GFAP-positive expression in both tumor cells and normal glial cells. The staining levels of the specimens in the fluorescence regions were higher than that in the non-fluorescence regions. GFAP expression was positive in 61 specimens and negative in 8 specimens. Comparison of Ki-67 proliferation index using chi-square test showed different fluorescence levels had different Ki-67 proliferation indexes . With high proliferation index of specimens, fluorescence level was brighter. WHO grade had no correlation with fluorescence levels . Conclusion: FLS-guided resection of MG is safe and effective. In the boundary area of MG, fluorescence levels and Ki-67 proliferation index showed correlation. FLS-guided resection achieved the function of 'reducing tumor cell,' thus reducing the proliferation index in the lesion area.收起

学科

【SCI学科】SURGERY;ONCOLOGY 【ESI学科】CLINICAL MEDICINE

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