Department of Cardiology, Taishan Medical College, Taian 271000 Shandong Province, China Department of Cardiology, Qilu Hospital, Shandong University, Jinan 250012 Shandong Province, China Laboratory of Molecular Biology, Shandong Academy of Medical Sciences, Jinan 250012 Shandong Province, China
刊名
Chinese Journal of Clinical Rehabilitation
年份
2005
卷号
Vol.9 No.39
页码
48-49
ISSN
1671-5926
摘要
Aim: To explore the relationship between gene mutation of methylenetetrahydrofolate reductase , which is the key enzyme of homocysteine metabolism, and coronary heart disease. Methods: 1 Between July 2001 and December 2003, totally 138 inpatients in the Department of Cardiology, Affiliated Hospital of Taishan Medical College were involved in the study, 33 patients without coronary artery disease were taken as the control group, and 105 patients with coronary heart disease were taken as coronary...更多
Aim: To explore the relationship between gene mutation of methylenetetrahydrofolate reductase , which is the key enzyme of homocysteine metabolism, and coronary heart disease. Methods: 1 Between July 2001 and December 2003, totally 138 inpatients in the Department of Cardiology, Affiliated Hospital of Taishan Medical College were involved in the study, 33 patients without coronary artery disease were taken as the control group, and 105 patients with coronary heart disease were taken as coronary heart disease group. They all knew the purpose of the study and agreed to participated in. 2 The genotype of methylenetetrahydrofolate reductase 677C→T was detected with polymerase chain reaction-restriction fragment length polymorphism . 3 The differences of the measurement data were compared with the t test. The percentages of different genotypes between the control group and coronary heart disease group were compared with the χ 2 test by using the 2×3 list. The total mutation rate between the two groups was compared with the fourfold table χ 2 test. The association between gene mutation and the attack of coronary heart disease was expressed with relative risk. Results: All the 33 patients without coronary artery dis ease and 105 patients with coronary heart disease were involved in the analysis of results. 1 In the control group, the homogenetic type was 18.2% , heterogenetic type was 36.4% , and the total mutation rate was 54.6%; In the coronary heart disease, the homogenetic type was 25.7% , heterogenetic type was 57.1% , and the total mutation rate was 82.8%. The percentages of the 3 genotypes between the two groups were obviously different . 2 The frequency of T allele was obviously higher in the coronary heart disease group than in the control group [36.4% , 54.3% , χ 2 =6.45, P < 0.05], the odds ratio of T allele to coronary heart disease was 2.08 . Conclusion: The methylenetetrahydrofolate reductase gene mut ation phenotype is an independent risk factor of coronary heart disease.收起
