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作者

Zhou, Q. Zhang, Y. Zhao, M. Wang, X. Ma, C. Jiang, X. Wu, T. Wang, D. Zhan, L.

作者单位

a Beijing Institute of Transfusion Medicine, Beijing key Laboratory of Blood Safety and Supply Technologies, Beijing, 100850, China Department of Blood Transfusion, PLA Army General Hospital, Beijing, 100700, China School of Public Health, Taishan Medical University, Taian, Shandong, 271000, China Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education), Nankai University, Tianjin, 300071, China

刊名

Scientific Reports

年份

2016

卷号

Vol.6

ISSN

2045-2322

摘要

Cryopreservation is critical in reducing redundant operations and also in quality control in dendritic cell therapy. Full maturation and efficient homing of DCs to T cell-region constitute a crucial aspect of DC immunotherapy; however, the in vivo migration and distribution pattern, as well as the anti-viral effect of DCs that matured from cryopreserved immature DCs remain to be revealed. In the present study, we compared cryoim-mDCs with DCs matured from fresh immature DCs in the aspects of ...更多

Cryopreservation is critical in reducing redundant operations and also in quality control in dendritic cell therapy. Full maturation and efficient homing of DCs to T cell-region constitute a crucial aspect of DC immunotherapy; however, the in vivo migration and distribution pattern, as well as the anti-viral effect of DCs that matured from cryopreserved immature DCs remain to be revealed. In the present study, we compared cryoim-mDCs with DCs matured from fresh immature DCs in the aspects of phenotypes, in vivo homing capacities as well as the anti-viral therapeutic effects to further clarify the effect of cryopreservation on DC-based cytotherapy. The results showed that cryopreservation impaired the homing ability of DCs which was associated with the reduced expression of CCR7 and disturbed cytoskeleton arrangement. Moreover, the antigen-specific CD8+ T cell response induced by cryoim-mDCs was much weaker than that induced by fmDCs in both the spleen and liver draining lymph nodes, which provided reduced protection from viral invasions. In conclusion, cryopreservation is a good method to keep the viability of immature DCs, however, the in vivo homing capacity and anti-viral therapeutic effect of DCs matured from frozen immature DCs were hindered to some extent.收起

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