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作者

Li, W.-H. Li, Y.-Z. Song, D.-D. Wang, X.-R. Liu, M. Wu, X.-D. Liu, X.-H.

作者单位

Department of Out-patient, Chinese PLA General Hospital, Beijing, China a Department of Pathophysiology, Chinese PLA General Hospital, Beijing, China State Key Laboratory of Kidney Disease (Chinese PLA General Hospital 2011DAV00088), Beijing, China Department of Pathophysiology, College of Basic Medical Sciences, Taishan Medical University, Taian, China

刊名

Microcirculation

年份

2014

卷号

Vol.21 No.6

页码

506-515

ISSN

1073-9688

关键词

Apoptosis Calreticulin Endoplasmic reticulum stress Endothelial cells Microwave radiation

摘要

Objective: This study was designed to evaluate whether exogenous CRT was beneficial for alleviating MR-induced injury by suppressing ER stress in rat MMECs. Methods: MMECs were pretreated with CRT for 12 hours, followed by the exposure to 2.856 GHz radiation at a mean power density of 30 mW/cm 2 for six minutes. MR-induced injury in MMECs was evaluated by LDH leakage, apoptosis, and cell viability analysis. The expression of GRP78, CRT, CHOP, Bcl-2, and Bax were examined by Western blot analys...更多

Objective: This study was designed to evaluate whether exogenous CRT was beneficial for alleviating MR-induced injury by suppressing ER stress in rat MMECs. Methods: MMECs were pretreated with CRT for 12 hours, followed by the exposure to 2.856 GHz radiation at a mean power density of 30 mW/cm 2 for six minutes. MR-induced injury in MMECs was evaluated by LDH leakage, apoptosis, and cell viability analysis. The expression of GRP78, CRT, CHOP, Bcl-2, and Bax were examined by Western blot analysis to reflect ER stress response and ER stress-related apoptosis. Results: MR induced marked MMECs injury, as shown by increased LDH leakage and apoptosis rate and decreased cell viability. MR also induced excessive ER stress, characterized by increased expression of GRP78 and CRT, and ER stress-related apoptotic signaling as well, as shown by the upregulation of CHOP and Bax and the downregulation of Bcl-2. Exogenous CRT pretreatment remarkably attenuated MR-induced cell apoptosis and LDH leakage, ER stress, and activation of the ER stress-related apoptotic signaling. Conclusions: Exogenous CRT attenuates MR-induced ER stress-related apoptosis by suppressing CHOP-mediated apoptotic signaling pathways in MMECs.收起

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