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作者

Liu, Y. Zhou, Y. Liu, L. Sun, L. Zhang, M. Li, D.

作者单位

a Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 61 DaiZong Street, Tai'an 271018, Shandong, China Department of Agronomy, Dezhou University, Dezhou, Shandong, 253023, China Taishan Medical University, Tai'an 271000, Shandong, China

刊名

Molecular Biology Reports

年份

2012

卷号

Vol.39 No.3

页码

2957-2966

ISSN

0301-4851

关键词

Maize Restriction-enzyme site Single-copy ZmMEK1

摘要

Mitogen-activated protein kinase cascade constitutes a conserved signaling module in eukaryotes. MAPK kinase plays a crucial role in a MAPK cascade. ZmMEK1 is the first characterized MAPKK gene in maize. Although ZmMEK1 has been studied in detail in biochemical level, the genomic organization of ZmMEK1 gene is obscure. In this research, we clarified ZmMEK1 is a single-copy gene in the maize genome. Southern blot analysis using 30 specific region of ZmMEK1 cDNA as a probe revealed the presence ...更多

Mitogen-activated protein kinase cascade constitutes a conserved signaling module in eukaryotes. MAPK kinase plays a crucial role in a MAPK cascade. ZmMEK1 is the first characterized MAPKK gene in maize. Although ZmMEK1 has been studied in detail in biochemical level, the genomic organization of ZmMEK1 gene is obscure. In this research, we clarified ZmMEK1 is a single-copy gene in the maize genome. Southern blot analysis using 30 specific region of ZmMEK1 cDNA as a probe revealed the presence of distinct single bands in each lane of EcoRI and HindIII. Although previous Southern blot analysis using full-length ZmMEK1 cDNA as a probe revealed several hybridizing bands, we showed here that all bands come from one genomic fragment corresponding to ZmMEK1 gene. Furthermore, ZmMEK1 was induced by PEG, abscisic acid , and salicylic acid and was down-regulated by NaCl.收起

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