A novel one- and two-photon fluorescent probe induced by light for selective imaging of Cys in living cells and tissues

作者: Ma, Y., Zhao, Y., Xia, L., Huang, J., Gu, Y., Wang, P.Email Author View Correspondence

作者单位: School of Pharmacy, Taishan Medical University, Chang Cheng Road, Taian, 271016, China a School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, China Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing, 210009, China

关键词: Engineering controlled terms Amino acids Cells Cytology Fluorescence spectroscopy Histology Integrated circuits Metal ions Metals pH Photons Photoreactivity Physiological models Probes Tissue Engineering uncontrolled terms Cysteine Fast response rate Fluorescent probes Limit of detection Low cytotoxicities Photoreactions Two photon Two-photon fluorescence imaging Engineering main heading Fluorescence imaging EMTREE drug terms amino acid antineoplastic agent coumarin cysteine fluorescent dye ic 2 metal unclassified drug coumarin derivative cysteine EMTREE medical terms antineoplastic activity Article cell membrane permeability conjugation controlled study cyclization drug cytotoxicity drug tolerance esterification fluorescence fluorescence imaging Hep-G2 cell line human human cell human tissue illumination isomerization light limit of detection MTT assay one photon fluorescent probe pH photoreactivity priority journal process development sensitivity analysis two photon fluorescent probe ultraviolet radiation animal chemical structure chemistry devices light molecular imaging mouse photon procedures sensitivity and specificity spectrofluorometry synthesis MeSH Animals Coumarins Cysteine Fluorescent Dyes Hep G2 Cells Humans Hydrogen-Ion Concentration Light Mice Molecular Imaging Molecular Structure Photons Sensitivity and Specificity

摘要: A novel one- and two-photon fluorescent probe based on a photoreaction was developed for the detection of cysteine . After it was reacted with Cys and illuminated by light, a strong turn-on fluorescence was observed at the excitation of 390 nm. The probe IC-2 was well tolerated at physiological pH conditions and exhibited a fast response rate to Cys within 10 min. Moreover, the probe IC-2 displayed high selectivity towards Cys among other amino acids and metal ions. Fluorescence response studies...更多; A novel one- and two-photon fluorescent probe based on a photoreaction was developed for the detection of cysteine . After it was reacted with Cys and illuminated by light, a strong turn-on fluorescence was observed at the excitation of 390 nm. The probe IC-2 was well tolerated at physiological pH conditions and exhibited a fast response rate to Cys within 10 min. Moreover, the probe IC-2 displayed high selectivity towards Cys among other amino acids and metal ions. Fluorescence response studies suggested that the limit of detection of IC-2 was calculated as 2.6 × 10 −8 M. An MTT assay demonstrated that IC-2 had good cell permeability and low cytotoxicity to HepG2 cells. Notably, IC-2 was successfully applied in one- and two-photon fluorescence imaging for Cys in living cells and tissues.收起