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作者

Pu Reum Sun Fei Fei Gao Hei Gwon Choi Wei Zhou Jae-Min Yuk Jaeyul Kwon Young-Ha Lee Guang-Ho Cha

作者单位

Infection Biology, Chungnam National University, School of Medicine, Daejeon 34134, Korea. Institute of Immunology, Taishan Medical College, Tai'an 271-000, Shandong, China. Department of Medical Science & Medical Education, Chungnam National University, School of Medicine, Daejeon 34134, Korea. Department of Medical Science &

刊名

The Korean journal of parasitology

年份

2019

卷号

Vol.57 No.2

页码

83-92

ISSN

0023-4001;1738-0006

关键词

ARPE-19 cell N-acetyl-L-cysteine T. gondii diphenyleneiodonium reactive oxygen species

摘要

Based on the reactive oxygen species regulatory properties of diphenyleneiodonium , we investigated the effects of DPI on host-infected T. gondii proliferation and determined specific concentration that inhibit the intracellular parasite growth but without severe toxic effect on human retinal pigment epithelial cells. As a result, it is observed that host superoxide, mitochondria superoxide and H2O2 levels can be increased by DPI, significantly, followed by suppression of T. gondii infection a...更多

Based on the reactive oxygen species regulatory properties of diphenyleneiodonium , we investigated the effects of DPI on host-infected T. gondii proliferation and determined specific concentration that inhibit the intracellular parasite growth but without severe toxic effect on human retinal pigment epithelial cells. As a result, it is observed that host superoxide, mitochondria superoxide and H2O2 levels can be increased by DPI, significantly, followed by suppression of T. gondii infection and proliferation. The involvement of ROS in anti-parasitic effect of DPI was confirmed by finding that DPI effect on T. gondii can be reversed by ROS scavengers, N-acetyl-L-cysteine and ascorbic acid. These results suggest that, in ARPE-19 cell, DPI can enhance host ROS generation to prevent T. gondii growth. Our study showed DPI is capable of suppressing T. gondii growth in host cells while minimizing the un-favorite side-effect to host cell. These results imply that DPI as a promising candidate material for novel drug development that can ameliorate toxoplasmosis based on ROS regulation.收起

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