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作者

Zhang, Li Zhang, Lei Li, Shancheng Zhang, Qin Luo, Yonggang Zhang, Chunhong Huan, Qin Zhang, Chunling

作者单位

Department of Health Care, Shandong Provincial Third Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250031, Shandong, China. Department of Nutrition, Shandong Provincial Third Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250031, Shandong, China. Office of big data, College of Medical Instruments, Shanghai University of Medicine & Health Sciences, Shanghai 203118, China. Department of Minimally invasive intervention, The Third Affiliated hospital of Shandong First Medical University, Jinan 250031, Shandong, China. Department of Intelligent Medical, Shandong Provincial Third Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250031, Shandong, China.

刊名

Canadian Journal of Physiology & Pharmacology

年份

2021

卷号

Vol.99 No.11

页码

1199-1206

ISSN

0008-4212

摘要

Circ-RBM4 has been revealed to have low expression in the renal tissues of diabetic nephropathy mice, and its underlying regulatory mechanism remains unexplored. The high glucose – treated mouse podocytes were used to establish DN cell models. A cell counting kit-8 assay was used to examine the viability of mouse podocytes. The expression of proteins related to fibrosis was detected using Western blot. The concentration of inflammation cytokines , IL-8) in mouse podocytes was assessed by ELI...更多

Circ-RBM4 has been revealed to have low expression in the renal tissues of diabetic nephropathy mice, and its underlying regulatory mechanism remains unexplored. The high glucose – treated mouse podocytes were used to establish DN cell models. A cell counting kit-8 assay was used to examine the viability of mouse podocytes. The expression of proteins related to fibrosis was detected using Western blot. The concentration of inflammation cytokines , IL-8) in mouse podocytes was assessed by ELISA. The interaction between genes was explored by luciferase reporter assays. HG treatment decreased the viability and elevated the expression of fibrosis and inflammation factors in mouse podocytes. Circ-RBM4 expression was downregulated in HG-treated mouse podocytes. Circ-RBM4 overexpression reversed HG-induced increase in levels of proteins related to fibrosis and the concentration of inflammation factors. The miR-153-3p was revealed to bind with circ-RBM4 and directly targeted nuclear factor I/C in mouse podocytes. Rescue assays indicated that circ-RBM4 attenuated HG-induced fibrosis and inflammation response in mouse podocytes by inhibiting miR-153-3p expression or upregulating NFIC expression. Circ-RBM4 alleviated the renal inflammation and renal fibrosis in DN by targeting the miR-153-3p/NFIC axis.收起

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