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作者

Xin, Yonghong Jia, Yanfei Cai, Yongjun Liu, Qiang Wang, Yunshan

作者单位

China National Heavy Duty Truck Group Corporation Hospital, Jinan 250031, Shandong Province, China Medical Laboratory and Diagnostic Center, Jinan Central Hospital, Shandong Key Laboratory of Tumor Target Molecule, Jinan 250013, Shandong Province, China Department of Blood Transfusion, The Fourth People's Hospital of Jinan, Taishan Medical College, Jinan 250031, Shandong Province, China

刊名

World Chinese Journal of Digestology

年份

2012

卷号

Vol.20 No.25

页码

2341

ISSN

1009-3079

摘要

AIM: To investigate the effect of inhibition of FUT3 gene expression with miRNAs on the proliferation of gastric cancer cells . METHODS: Vectors carrying two miRNAstargeting the FUT3 gene were constructed andtransiently transfected into KATO-III cells using lipidosome-mediated method. RT-PCR was performed to detect the expression of FUT3 mRNA, and immunocytochemistry and flow cytometry analysis were carried out to test expression variation of sLeA antigen. MTT assay and colony-forming assay were...更多

AIM: To investigate the effect of inhibition of FUT3 gene expression with miRNAs on the proliferation of gastric cancer cells . METHODS: Vectors carrying two miRNAstargeting the FUT3 gene were constructed andtransiently transfected into KATO-III cells using lipidosome-mediated method. RT-PCR was performed to detect the expression of FUT3 mRNA, and immunocytochemistry and flow cytometry analysis were carried out to test expression variation of sLeA antigen. MTT assay and colony-forming assay were used to analyze cell proliferation and to detect the effect of decreased FUT3 expression on cell growth. RESULTS: Compared to non-transfected cells and cells transfected with empty vector, the relative expression levels of FUT3 mRNA were significantly decreased ; the sLeA antigen expression levels were also significantly reduced ; cell growth was significantly inhibited ; and colony-forming ability was significantly reduced in FUT3-miRNA and FUT3- miRNA2 transfeced cells. CONCLUSION: Transfection of miRNAs targeting the FUT3 gene can effectively inhibit the proliferation of KATO-III cells.收起

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