China National Heavy Duty Truck Group Corporation Hospital, Jinan 250031, Shandong Province, ChinaMedical Laboratory and Diagnostic Center, Jinan Central Hospital, Shandong Key Laboratory of Tumor Target Molecule, Jinan 250013, Shandong Province, ChinaDepartment of Blood Transfusion, The Fourth People's Hospital of Jinan, Taishan Medical College, Jinan 250031, Shandong Province, China
刊名
World Chinese Journal of Digestology
年份
2012
卷号
Vol.20 No.25
页码
2341
ISSN
1009-3079
摘要
AIM: To investigate the effect of inhibition of FUT3 gene expression with miRNAs on the proliferation of gastric cancer cells . METHODS: Vectors carrying two miRNAstargeting the FUT3 gene were constructed andtransiently transfected into KATO-III cells using lipidosome-mediated method. RT-PCR was performed to detect the expression of FUT3 mRNA, and immunocytochemistry and flow cytometry analysis were carried out to test expression variation of sLeA antigen. MTT assay and colony-forming assay were...更多
AIM: To investigate the effect of inhibition of FUT3 gene expression with miRNAs on the proliferation of gastric cancer cells . METHODS: Vectors carrying two miRNAstargeting the FUT3 gene were constructed andtransiently transfected into KATO-III cells using lipidosome-mediated method. RT-PCR was performed to detect the expression of FUT3 mRNA, and immunocytochemistry and flow cytometry analysis were carried out to test expression variation of sLeA antigen. MTT assay and colony-forming assay were used to analyze cell proliferation and to detect the effect of decreased FUT3 expression on cell growth. RESULTS: Compared to non-transfected cells and cells transfected with empty vector, the relative expression levels of FUT3 mRNA were significantly decreased ; the sLeA antigen expression levels were also significantly reduced ; cell growth was significantly inhibited ; and colony-forming ability was significantly reduced in FUT3-miRNA and FUT3- miRNA2 transfeced cells. CONCLUSION: Transfection of miRNAs targeting the FUT3 gene can effectively inhibit the proliferation of KATO-III cells.收起
