A highly sensitive NIR fluorescence probe for hypoxia imaging in cells and ulcerative colitis

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作者: Sheng Wang, Xiao-Fan Zhang, He-Song Wang, Jing Liu, Shi-Li Shen, Xiao-Qun Cao

作者单位: Affiliations ¹ Institute of Optical Functional Materials for Biomedical Imaging, School of Chemistry and Pharmaceutical Engineering, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, Shandong, 271016, PR China. ² Department of Gastroenterology, Medical Research Center, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, Shandong, 250014, PR China. Electronic address: 2307@sdhospital.com.cn. ³ Institute of Optical Functional Materials for Biomedical Imaging, School of Chemistry and Pharmaceutical Engineering, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, Shandong, 271016, PR China. Electronic address: slshen@sdfmu.edu.cn. ⁴ Institute of Optical Functional Materials for Biomedical Imaging, School of Chemistry and Pharmaceutical Engineering, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, Shandong, 271016, PR China. Electronic address: xqcao@sdfmu.edu.cn.

关键词: Hypoxia imaging Near-infrared fluorescence Nitroreductase Tumor Ulcerative colitis.

摘要: Ulcerative colitis, a kind of inflammatory bowel disease , is caused by dysregulated immune response of intestinal bacteria. This chronic disorder can lead to a deficiency of O in the colon microenvironment. Nitroreductase is a highly expressed endogenous enzyme under hypoxia, so the detection of NTR can provide diagnostic information about ulcerative colitis. Herein, an ultrasensitive NTR-triggered fluorescence probe is developed for hypoxia imaging in ulcerative colitis. The probe shows a s...更多; Ulcerative colitis, a kind of inflammatory bowel disease , is caused by dysregulated immune response of intestinal bacteria. This chronic disorder can lead to a deficiency of O in the colon microenvironment. Nitroreductase is a highly expressed endogenous enzyme under hypoxia, so the detection of NTR can provide diagnostic information about ulcerative colitis. Herein, an ultrasensitive NTR-triggered fluorescence probe is developed for hypoxia imaging in ulcerative colitis. The probe shows a significant fluorescence enhancement after reacting with NTR, with an extremely low detection limit of 0.096 ng/mL. Furthermore, we apply it for fluorescence imaging of hypoxia in living cells, tumors and dextran sulphate sodium -induced ulcerative colitis mouse models. We believe that the probe may be investigated as an effective potential tool for gaining insight into the hypoxia-relevant diseases, such as cancer and ulcerative colitis.收起

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