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作者

Lei Liu, Taiping Li, Hui Dong, Xijun Wang

作者单位

Affiliations ¹ GAP Research Center of Chinese Medicine, Chinmedomics Research Center of State Administration of TCM, Heilongjiang University of Chinese Medicine, Harbin 150040, China. ² School of Parmacy and Pharmaceutical Sciences & Institute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250100, China.

刊名

Molecules (Basel, Switzerland)

年份

2022

卷号

Vol.27 No.20

页码

7014

ISSN

1420-3049

关键词

Paeoniae Radix Alba amino acid metabolism kaempferol metabolomics paeoniflorin rheumatoid arthritis.

摘要

Objective: The mechanism of action and potential targets of Paeoniae RadixAlba in the treatment of adjuvant-induced arthritis rats are explained using metabolomics and network pharmacology techniques, and the research evidence for the development of anti-rheumatoid arthritis drugs is enriched. Methods: The rats were injected with Freund's complete adjuvant to induce arthritis. We then measured the general physical characteristics, examined their X-rays and histopathology to evaluate the path...更多

Objective: The mechanism of action and potential targets of Paeoniae RadixAlba in the treatment of adjuvant-induced arthritis rats are explained using metabolomics and network pharmacology techniques, and the research evidence for the development of anti-rheumatoid arthritis drugs is enriched. Methods: The rats were injected with Freund's complete adjuvant to induce arthritis. We then measured the general physical characteristics, examined their X-rays and histopathology to evaluate the pathological condition of the inflammation models, and conducted metabolomics studies on the change in urine metabolism caused by CFA. The lyophilized powder of B at a dose of 2.16 g/kg was orally administered to the rats continuously for 28 days, and the therapeutic effect was evaluated. Network pharmacology prediction shows that B contains the target action of the ingredient, and the simulation of the target molecular docking, in combination with the metabolomics analysis results, shows that B has a potential role in the treatment of AIA rats. Results: B can reduce the paw swelling and pathological changes in rats caused by CFA, reverse the levels of 12 urine biomarkers, and regulate histidine metabolism, phenylalanine metabolism, arginine, proline metabolism, pyrimidine metabolism, etc. The prediction of the active ingredient target in B indicates that it may act as an inflammatory signaling pathway in anti-RA, among them being paeoniflorin, palbinone, beta-sitosterol, kaempferol, and catechin, which are the significant active ingredients. Conclusion: The metabolomics results revealed the markers and metabolic mechanisms of urinary metabolic disorders in rats with AIA, demonstrated the efficacy of the therapeutic effect of B, and identified the key ingredients in B, providing theoretical support for the subsequent development and utilization of B.收起

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