获取途径

作者

Yanrong Zhang Jing Wang Sara Natasha Ghobadi Haiyan Zhou Ai Huang Marco Gerosa Qingyi Hou Olivier Keunen Anna Golebiewska Frezghi G Habte Gerald A Grant Ramasamy Paulmurugan Kevin S Lee Max Wintermark

作者单位

Acupuncture and Tuina School/Third Teaching Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China. ⁴ Department of Radiology, Neuroradiology Division, Stanford University, Stanford, CA, USA Department of Nuclear Medicine, Guangdong Provincial People's Hospital, Guangzhou, China. ⁷ In Vivo Imaging Facility, Luxembourg Institute of Health, Luxembourg. ⁸ Department of Oncology, Luxembourg Institute of Health, Luxembourg. ⁹ Department of Radiology, Molecular Imaging Program at Stanford, Stanford, CA, USA. ¹⁰ Division of Pediatric Neurosurgery, Department of Neurosurgery, Lucile Packard Children's Hospital, Stanford University School of Medicine, Stanford, CA, USA. ¹¹ Molecular Imaging Program at Stanford (MIPS), Canary Center for Cancer Early Detection, Department of Radiology, Stanford University, Stanford, CA, USA. ¹² Departments of Neuroscience and Neurosurgery and Center for Brain, Immunology, and Glia, School of Medicine, University of Virginia, Charlottesville, VA, USA. ¹³ Department of Neuroradiology, University of Texas MD Anderson Cancer Center, Houston, TX, USA. Electronic address: Max.Wintermark@gmail.com. Affiliations ¹ Department of Radiology, Neuroradiology Division, Stanford University, Stanford, CA, USA. ² Department of Radiology, Neuroradiology Division, Stanford University, Stanford, CA, USA Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. ⁵ Department of Radiology, Neuroradiology Division, Stanford University, Stanford, CA, USA Department of Radiology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China. ³ Department of Radiology, Neuroradiology Division, Stanford University, Stanford, CA, USA Department of Diagnostic and Public Health, University of Verona, Verona, Italy. ⁶ Department of Radiology, Neuroradiology Division, Stanford University, Stanford, CA, USA

刊名

Ultrasound in medicine & biology

年份

2023

ISSN

1879-291X

关键词

Focused ultrasound Glioblastoma Magnetic resonance imaging–guided focused ultrasound Microbubbles Microglia and macrophage activation Sterile inflammation Tumor-associated macrophages and microglia.

摘要

An orthotopically allografted mouse GL26 glioma model was used to evaluate the effect of transient, focal opening of the blood-brain barrier on the composition of tumor-associated macrophages and microglia . BBB opening was induced by magnetic resonance imaging -guided focused ultrasound combined with microbubbles. CX3CR1-GFP cells and CCR2-RFP cells in brain tumors were quantified in microscopic images. Tumors in animals treated with a single session of MRgFUS did not exhibit significant cha...更多

An orthotopically allografted mouse GL26 glioma model was used to evaluate the effect of transient, focal opening of the blood-brain barrier on the composition of tumor-associated macrophages and microglia . BBB opening was induced by magnetic resonance imaging -guided focused ultrasound combined with microbubbles. CX3CR1-GFP cells and CCR2-RFP cells in brain tumors were quantified in microscopic images. Tumors in animals treated with a single session of MRgFUS did not exhibit significant changes in cell numbers when compared with tumors in animals not receiving FUS. However, tumors that received two or three sessions of MRgFUS had significantly increased amounts of both CX3CR1-GFP and CCR2-RFP cells. The effect of MRgFUS on immune cell composition was also characterized and quantified using flow cytometry. Glioma implantation resulted in increased amounts of lymphocytes, monocytes and neutrophils in the brain parenchyma. Tumors administered MRgFUS exhibited increased numbers of monocytes and monocyte-derived TAMs. In addition, MRgFUS-treated tumors exhibited more CD80+ cells in monocytes and microglia. In summary, transient, focal opening of the BBB using MRgFUS combined with microbubbles can activate the homing and differentiation of monocytes and induce a shift toward a more pro-inflammatory status of the immune environment in glioblastoma.收起

文献类型

期刊