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作者

Xudong Zhao Fengyun Su Fanhua Kong Qing Guo Xiuzhu Wang Hong Cui Qinwen Li Wangmeng Zhang Lei Li Aihua Li

作者单位

Affiliations ¹ Department of Obstetrics and Gynaecology, Liao Cheng People's Hospital, Liaocheng, China. ² Department of Obstetrics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China. ³ Department of Obstetrics, The Affiliated Taian City Central Hospital of Qingdao University, Taian, China. ⁴ Department of Pharmacy, The Second Affiliated Hospital of Shandong First Medical University, Taian, China. ⁵ Departments of Thoracic Surgery, The Affiliated Taian City Central Hospital of Qingdao University, Taian, China. ⁶ Departments of Critical Care Medicine, The Affiliated Taian City Central Hospital of Qingdao University, Taian, China. ⁷ Department of Obstetrics and Gynecology Color Ultrasound, The Affiliated Taian City Central Hospital of Qingdao University, Taian, China. ⁸ Department of Obstetrics, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.

刊名

The journal of obstetrics and gynaecology research

卷号

Vol.49 No.1

页码

141-153

ISSN

1447-0756

关键词

WD repeat domain 5 blood pressure microRNA-101-3p preeclampsia trophoblast urine protein.

摘要

Background: Decreased proliferation and invasion of trophoblast were proven to be involved in the pathogenesis of preeclampsia . However, the regulatory network has not been clarified yet. This study aimed to explore the role of miR-101-3p in the progression of PE. Methods: miR-101-3p expression in placentas of pregnant women with or without PE was analyzed by real-time quantitative PCR . Trophoblastic HTR-8/SVneo and HPT-8 cell lines were cultured and underwent hypoxia/reoxygenation treatment ...更多

Background: Decreased proliferation and invasion of trophoblast were proven to be involved in the pathogenesis of preeclampsia . However, the regulatory network has not been clarified yet. This study aimed to explore the role of miR-101-3p in the progression of PE. Methods: miR-101-3p expression in placentas of pregnant women with or without PE was analyzed by real-time quantitative PCR . Trophoblastic HTR-8/SVneo and HPT-8 cell lines were cultured and underwent hypoxia/reoxygenation treatment to mimic PE in vitro. Cell proliferation and invasion were analyzed in gain-of and loss-of-function assays. Finally, we undertook in vivo studies to explore effects of miR-101-3p in the PE model. Results: Compared to placentas from patients without PE, miR-101-3p expressed significantly higher in placentas from PE patients, and its level was positively correlated with the severity of patients. In vitro studies found that overexpression of miR-101-3p significantly suppressed cell proliferation and invasion, while knockdown of miR-101-3p reversed the impacts of H/R treatment. Further research showed that the expression of WD repeat domain 5 was significantly lower in placentas from patients with PE, and its level was negatively associated with the severity of patients. In vitro and in vivo studies confirmed that miR-101-3p promoted PE progression through the regulation of WD WDR5 expression. Conclusion: Increased expression of miR-101-3p in placenta contributes to the development of PE by suppressing WDR5-mediated proliferation and invasion of trophoblast.收起

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