Affiliations 1 Department of Obstetrics and Gynaecology, Liao Cheng People's Hospital, Liaocheng, China. 2 Department of Obstetrics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China. 3 Department of Obstetrics, The Affiliated Taian City Central Hospital of Qingdao University, Taian, China. 4 Department of Pharmacy, The Second Affiliated Hospital of Shandong First Medical University, Taian, China. 5 Departments of Thoracic Surgery, The Affiliated Taian City Central Hospital of Qingdao University, Taian, China. 6 Departments of Critical Care Medicine, The Affiliated Taian City Central Hospital of Qingdao University, Taian, China. 7 Department of Obstetrics and Gynecology Color Ultrasound, The Affiliated Taian City Central Hospital of Qingdao University, Taian, China. 8 Department of Obstetrics, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.
刊名
The journal of obstetrics and gynaecology research
Background: Decreased proliferation and invasion of trophoblast were proven to be involved in the pathogenesis of preeclampsia . However, the regulatory network has not been clarified yet. This study aimed to explore the role of miR-101-3p in the progression of PE. Methods: miR-101-3p expression in placentas of pregnant women with or without PE was analyzed by real-time quantitative PCR . Trophoblastic HTR-8/SVneo and HPT-8 cell lines were cultured and underwent hypoxia/reoxygenation treatment ...更多
Background: Decreased proliferation and invasion of trophoblast were proven to be involved in the pathogenesis of preeclampsia . However, the regulatory network has not been clarified yet. This study aimed to explore the role of miR-101-3p in the progression of PE. Methods: miR-101-3p expression in placentas of pregnant women with or without PE was analyzed by real-time quantitative PCR . Trophoblastic HTR-8/SVneo and HPT-8 cell lines were cultured and underwent hypoxia/reoxygenation treatment to mimic PE in vitro. Cell proliferation and invasion were analyzed in gain-of and loss-of-function assays. Finally, we undertook in vivo studies to explore effects of miR-101-3p in the PE model. Results: Compared to placentas from patients without PE, miR-101-3p expressed significantly higher in placentas from PE patients, and its level was positively correlated with the severity of patients. In vitro studies found that overexpression of miR-101-3p significantly suppressed cell proliferation and invasion, while knockdown of miR-101-3p reversed the impacts of H/R treatment. Further research showed that the expression of WD repeat domain 5 was significantly lower in placentas from patients with PE, and its level was negatively associated with the severity of patients. In vitro and in vivo studies confirmed that miR-101-3p promoted PE progression through the regulation of WD WDR5 expression. Conclusion: Increased expression of miR-101-3p in placenta contributes to the development of PE by suppressing WDR5-mediated proliferation and invasion of trophoblast.收起
