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作者

Xiaosan Tang Heng Li Baozeng An Haibo Ma Nana Huang Xiaohong Li

作者单位

Affiliations ¹ Department of Neurology, Central Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250031, China. ² Department of Psychology, Central Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250031, China. ³ Department of Neurology, Shandong Provincial Third Hospital, Shandong University, Jinan, Shandong 250031, China. ⁴ Department of Neurology, Central Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250031, China. Electronic address: xuanhuangchen@tom.com.

刊名

Transplant immunology

年份

2022

卷号

Vol.75

页码

101701

ISSN

1878-5492

关键词

Parkinson's disease Rat model Stem cells Transplantation Umbilical cord blood.

摘要

Earlier published research showed that cord blood-derived multipotent stem cells exhibited the intrinsic expression of specific transcription factors and seems to be induced to form dopamine neurons in vitro. In this research, we further investigated the therapeutic potential of CB-SCs in 6-hydroxydopamine lesioned Parkinson's disease rats. The results of PCR analysis showed that CB-SCs could express transcription factors associated with pluripotentiality and dopaminergic differentiation . Af...更多

Earlier published research showed that cord blood-derived multipotent stem cells exhibited the intrinsic expression of specific transcription factors and seems to be induced to form dopamine neurons in vitro. In this research, we further investigated the therapeutic potential of CB-SCs in 6-hydroxydopamine lesioned Parkinson's disease rats. The results of PCR analysis showed that CB-SCs could express transcription factors associated with pluripotentiality and dopaminergic differentiation . After being transplanted into the striatum and substantia nigra of PD rats, most of CB-SCs developed a fate commitment to dopaminergic differentiation, expressed as the expression of tyrosine hydroxylase and dopamine transporter . The improvement effect of cell transplantation on dyskinesia in PD rats was better than that in sham control group. Moreover, higher levels of TH protein in brain homogenates further demonstrated that there were more surviving dopamine neurons in the brain of transplanted PD rats. Study concluds, CB SCS transplantation could promote the regeneration of dopamine neurons and behavioral recovery of PD rats.收起

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