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作者

Fengyi Wang Yaqi Liu Lu Zhang Zuhao Zhang Chuan Huang Dejin Zang Haiqing Wang Shenguang Ge Jinghua Yu

作者单位

²F. W. and Y. L. contributed equally to this paper. ⁴School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China ¹Institute for Advanced Interdisciplinary Research, University of Jinan, Jinan, 250022, PR China ³Institute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, 250117, PR China

刊名

Analytica Chimica Acta

年份

2022

卷号

Vol.1226

页码

340265

ISSN

0003-2670

关键词

Photoelectrochemical Tripodal DNA walker h-BN nanosheets CdS quantum Dots MicroRNA-141

摘要

Herein, a high-sensitivity photoelectrochemical biosensor was developed based on CdS quantum dots sensitized porous hexagonal boron nitride nanosheets and the multiple sites tripodal DNA walker formed by catalytic hairpin assembly . Noticeably, the porous structure of h-BN NSs gives it a lasting gift of large specific surface areas and extensive active reaction sites, which makes it possible to be employed as photoelectric substrate material. The h-BN/CdS QDs composite material promotes the...更多

Herein, a high-sensitivity photoelectrochemical biosensor was developed based on CdS quantum dots sensitized porous hexagonal boron nitride nanosheets and the multiple sites tripodal DNA walker formed by catalytic hairpin assembly . Noticeably, the porous structure of h-BN NSs gives it a lasting gift of large specific surface areas and extensive active reaction sites, which makes it possible to be employed as photoelectric substrate material. The h-BN/CdS QDs composite material promotes the transmission of photogenerated electrons and holes, the outstanding photoelectric conversion efficiency. Meanwhile, the CHA-formed TDWs triggered by miRNA-141 moved on track strand-functionalized electrode, so that a large number of alkaline phosphatase was immobilized on the electrode surface and further in situ catalyzed ascorbic acid 2-phosphate to produce ascorbic acid as the electron donor. As the result of the decisive influence of electron donor on PEC biosensor, the sensitive detection of miRNA-141 was realized. The proposed PEC biosensor displayed an excellent linear relationship ranging from 1 fM to 100 nM with a detection limit of 0.73 fM, providing a powerful strategy for early clinical diagnosis and biomedical research.收起

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