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作者

Shengying Zhou Xingchen Ding Yiyuan Zhang Yuanyuan Liu Xiaowen Wang Yujiao Guo Jianguang Zhang Xiao Liu Guanzhong Gong Ya Su Lizhen Wang Miaoqing Zhao null Man Hu

作者单位

⁴Shandong University cancer center, Jinan, 250100, Shandong, China ²Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, NO.440 Ji Yan Road, Jinan, 250117, Shandong, China ⁷960 Hospital of the Joint Logistics Support Force of the Chinese People’s Liberation Army, Jinan, 250031, Shandong, China ³Department of Pathology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, 250117, Shandong, China ⁵Affiliated Hospital of Jining Medical College, Jining, 272067, Shandong, China ⁶Zibo Wanjie Cancer Hospital, Zibo, 255202, Shandong, China ¹School of Clinical Medicine, Shandong Second Medical University, Weifang, 261053, Shandong, China

刊名

Scientific Reports

年份

2024

卷号

Vol.14

关键词

Relative biological effectiveness Proton beam therapy Hippocampus Neuronal damage Radiation-induced brain injury

摘要

The study aimed to determine the specific relative biological effectiveness of various cells in the hippocampus following proton irradiation. Sixty Sprague–Dawley rats were randomly allocated to 5 groups receiving 20 or 30 Gy of proton or photon irradiation. Pathomorphological neuronal damage in the hippocampus was assessed using Hematoxylin–eosin staining. The expression level of NeuN, Nestin, Caspase-3, Olig2, CD68 and CD45 were determined by immunohistochemistry . The RBE range established ...更多

The study aimed to determine the specific relative biological effectiveness of various cells in the hippocampus following proton irradiation. Sixty Sprague–Dawley rats were randomly allocated to 5 groups receiving 20 or 30 Gy of proton or photon irradiation. Pathomorphological neuronal damage in the hippocampus was assessed using Hematoxylin–eosin staining. The expression level of NeuN, Nestin, Caspase-3, Olig2, CD68 and CD45 were determined by immunohistochemistry . The RBE range established by comparing the effects of proton and photon irradiation at equivalent biological outcomes. Proton induced more severe damage to neurons than photon, but showed no difference compared to photon. The RBE of neuron was determined to be 1.65. Similarly, both proton and proton resulted in more inhibition of oligodendrocytes and activation of microglia in the hippocampal regions than photon and photon. However, the expression of Olig2 was higher and CD68 was lower in the proton group than in the photon group. The RBE of oligodendrocyte and microglia was estimated to be between 1.1 to 1.65. For neural stem cells and immune cells, there were no significant difference in the expression of Nestin and CD45 between proton and photon irradiation . Therefore, the RBE for NSCs and immune cell was determined to be 1.1. These findings highlight the varying RBE values of different cells in the hippocampus in vivo. Moreover, the actual RBE of the hippocampus may be higher than 1.1, suggesting that using as RBE value of 1.1 in clinical practice may underestimate the toxicities induced by proton radiation.收起

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