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作者

Wang, Sheng Zhang, Xiao-Fan Wang, He-Song Liu, Jing Shen, Shi-Li Cao, Xiao-Qun

作者单位

¹Institute of Optical Functional Materials for Biomedical Imaging, School of Chemistry and Pharmaceutical Engineering, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, Shandong, 271016, PR China ²Department of Gastroenterology, Medical Research Center, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, Shandong, 250014, PR China

刊名

Talanta

年份

2023

卷号

Vol.252

ISSN

0039-9140

关键词

Hypoxia imaging Near-infrared fluorescence Nitroreductase Tumor Ulcerative colitis

摘要

Ulcerative colitis, a kind of inflammatory bowel disease , is caused by dysregulated immune response of intestinal bacteria. This chronic disorder can lead to a deficiency of O 2 in the colon microenvironment. Nitroreductase is a highly expressed endogenous enzyme under hypoxia, so the detection of NTR can provide diagnostic information about ulcerative colitis. Herein, an ultrasensitive NTR-triggered fluorescence probe is developed for hypoxia imaging in ulcerative colitis. The probe shows a...更多

Ulcerative colitis, a kind of inflammatory bowel disease , is caused by dysregulated immune response of intestinal bacteria. This chronic disorder can lead to a deficiency of O 2 in the colon microenvironment. Nitroreductase is a highly expressed endogenous enzyme under hypoxia, so the detection of NTR can provide diagnostic information about ulcerative colitis. Herein, an ultrasensitive NTR-triggered fluorescence probe is developed for hypoxia imaging in ulcerative colitis. The probe shows a significant fluorescence enhancement after reacting with NTR, with an extremely low detection limit of 0.096 ng/mL. Furthermore, we apply it for fluorescence imaging of hypoxia in living cells, tumors and dextran sulphate sodium -induced ulcerative colitis mouse models. We believe that the probe may be investigated as an effective potential tool for gaining insight into the hypoxia-relevant diseases, such as cancer and ulcerative colitis. [Display omitted] • An ultrasensitive NIR fluorescence probe for NTR is developed with a low detection limit of 0.096 ng/mL. • The probe can be used to detect hypoxia in living cells. • The probe is applied for the visualization of NTR in hypoxic tumors. • In vivo accurate imaging of hypoxia is realized in ulcerative colitis mouse model.收起

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