A mitochondria-targeted ratiometric fluorescent probe for hypochlorite and its applications in bioimaging

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作者: Shi-Li Shen Xuan Zhao Xiao-Fan Zhang Xuan-Li Liu Hao Wang Yi-Ying Dai Jun-Ying Miao Bao-Xiang Zhao

作者单位: School of Chemistry and Pharmaceutical Engineering, Taishan Medical University, Taian 271016, P. R. China a School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P. R. China E-mail: bxzhao@sdu.edu.cn Fax: +86 531 88564464 Tel: +86 531 88366425 School of Life Science, Shandong University, Jinan 250100, P. R. China

摘要: A novel ratiometric probe (RCP) for −OCl was developed based on the fluorescence resonance energy transfer (FRET) platform. The probe was constructed by integrating the coumarin moiety (FRET donor) with the rhodamine moiety (FRET acceptor). Upon treatment with −OCl, the coumarin emission at 483 nm decreased and the rhodamine emission at 570 nm increased, enabling the probe to provide accurate detection of −OCl (in the concentration range of 0–50 μM). The probe exhibited brilliant selectivity and...更多; A novel ratiometric probe (RCP) for −OCl was developed based on the fluorescence resonance energy transfer (FRET) platform. The probe was constructed by integrating the coumarin moiety (FRET donor) with the rhodamine moiety (FRET acceptor). Upon treatment with −OCl, the coumarin emission at 483 nm decreased and the rhodamine emission at 570 nm increased, enabling the probe to provide accurate detection of −OCl (in the concentration range of 0–50 μM). The probe exhibited brilliant selectivity and sensitivity, rapid response and low cytotoxicity. More importantly, the introduction of the quaternized pyridine moiety can not only manage to increase the solubility, but also achieve mitochondria-targeting. The probe was applied successfully to imaging endogenous −OCl in mitochondria, highlighting its potential applications in bioanalysis.收起

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