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作者

Jin-shan Yang Bao-jian Li Hua-wei Lu Yu Chen Chuan Lu Rui-xia Zhu Si-hai Liu Qing-ting Yi Jing Li Chun-hui Song

作者单位

Department of Thoracic Surgery, Central Hospital of Zaozhuang Mineral Group, Zaozhuang, 277800, China Taishan Medical University, Taian, 271000, China Department of Oncology, People’s Hospital of Xuecheng, Zaozhuang, 277000, China Department of Oncology, Central Hospital of Zaozhuang Mineral Group, Qilianshan Road, Zaozhuang, 277800, China

刊名

Tumor Biology

年份

2015

卷号

Vol.36 No.4

页码

3035-3042

ISSN

1010-4283

关键词

MicroRNAs miR-148a miR-148b miR-152 miR-21 Serum Non-small cell lung cancer Screening

摘要

Lung cancer, predominantly by non-small cell lung cancer (NSCLC), is the leading cause of cancer-related deaths over the world. Late diagnosis is one of important reasons for high mortality rate in lung cancer. Current diagnostic approaches have disadvantages such as low accuracy, high cost, invasive procedure, etc. MicroRNAs were previously proposed as promising novel biomarkers in cancer screening. In this study, we evaluated the predictive power of four candidate miRNAs in NSCLC detection. Ou...更多

Lung cancer, predominantly by non-small cell lung cancer (NSCLC), is the leading cause of cancer-related deaths over the world. Late diagnosis is one of important reasons for high mortality rate in lung cancer. Current diagnostic approaches have disadvantages such as low accuracy, high cost, invasive procedure, etc. MicroRNAs were previously proposed as promising novel biomarkers in cancer screening. In this study, we evaluated the predictive power of four candidate miRNAs in NSCLC detection. Our study involved 152 NSCLC patients and 300 healthy controls. Blood samples were obtained from the total 452 subjects. After miRNA extraction from serum, the expression of miRNAs in cases and controls were quantified by qRT-PCR and normalized to the level of U6 small RNA. Statistical analyses were performed to compare miRNA levels between cases and controls. Stratified analyses were employed to compare miRNA levels in NSCLC patients with different clinical characteristics. Serum miR-148a, miR-148b, and miR-152 were significantly downregulated in NSCLC patients. However, overexpression of serum miR-21 was observed in NSCLC patients. The combination of four candidate miRNAs exhibited the highest predictive accuracy in NSCLC screening compared with individual miRNAs (AUC = 0.97). Low level of miRNA-148/152 members may associate with advanced stage, large tumor size, malignant cell differentiation, and metastasis. High expression of miR-21 was possibly correlated with large size tumor and advanced cancer stage. Our results showed the dysregulation of miR-148/152 family and miR-21 in NSCLC patients. Hence, the four candidate miRNAs have great potential to serve as promising novel biomarkers in NSCLC screening. Further large-scale studies are needed to validate our results.收起

学科

【SCI学科】ONCOLOGY 【ESI学科】CLINICAL MEDICINE

影响因子

2016年:3.65;2015年:2.926;2014年:3.611;2013年:2.84;2012年:2.518

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期刊

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