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作者

Guo, S Gao, Q Jiao, Q Hao, W Gao, X Cao, JM

作者单位

Taishan Med Coll, Dept Radiol, Tai An 271016, Shandong, Peoples R China   增强组织信息的名称     Taishan Medical University Chinese Acad Med Sci, Peking Union Med Coll, Inst Basic Med Sci, Dept Physiol & Pathophysiol,Sch Basic Med, Beijing 100005, Peoples R China   增强组织信息的名称     Institute of Basic Medical Sciences - CAMS     Chinese Academy of Medical Sciences - Peking Union Medical College

刊名

World Journal of Gastroenterology

年份

2012

卷号

Vol.18 No.24

页码

3145-3155

ISSN

1007-9327;2219-2840

关键词

Growth substances Gastric ulcer Stress Behavior and emotions Autonomic nerve Heart rate variability

分类号

616.3

摘要

AIM: To investigate the mechanism of gastric mucosal demage induced by water immersion restraint stress (WRS) and its prevention by growth hormone releasing peptide-6 (GHRP-6). METHODS: Male Wistar rats were subjected to conscious or unconscious (anesthetized) WRS, simple restraint (SR), free swimming (FS), non-water fluid immersion, immersion without water contact, or rats were placed in a cage surrounded by sand. To explore the sensitivity structures that influence the stress reaction besides ...更多

AIM: To investigate the mechanism of gastric mucosal demage induced by water immersion restraint stress (WRS) and its prevention by growth hormone releasing peptide-6 (GHRP-6). METHODS: Male Wistar rats were subjected to conscious or unconscious (anesthetized) WRS, simple restraint (SR), free swimming (FS), non-water fluid immersion, immersion without water contact, or rats were placed in a cage surrounded by sand. To explore the sensitivity structures that influence the stress reaction besides skin stimuli, a group the rats had their eyes occluded. Cervical bilateral trunk vagotomy or atropine injection was performed in some rats to assess the parasympathetic role in mucosal damage. Gastric mucosal lesions, acid output and heart rate variability were measured. Plasma renin, endothelin-1 and thromboxane B2 and gastric heat shock protein 70 were also assayed. GHRP-6 was injected [intraperitoneal (IP) or intracerebroventricular (ICV)] 2 h before the onset of stress to observe its potential prevention of the mucosal lesion. RESULTS: WRS for 6 h induced serious gastric mucosal lesion [lesion area, WRS 81.8 ± 6.4 mm?vs normal control 0.0 ± 0.0 mm, P ?vs WRS at 23?°C 81.8 ± 6.4 mm, P < 0.01) and was consciousness-dependent. The injury could not be prevented by eye occlusion, but could be prevented by avoiding contact of the rat body with the water by dressing it in an impermeable plastic suit. When water was replaced by vegetable oil or liquid paraffin, there were gastric lesions in the same grade of water immersion. When rat were placed in a cage surrounded by sand, there were no gastric lesions. All these data point to a remarkable importance of cutenuous information transmitted to the high neural center that by vagal nerves reaching the gastric mucosa. FS alone also induced serious gastric injury, but SR could not induce gastric injury. Bilateral vagotomy or atropine prevented the WRS-induced mucosal lesion, indicating that increased outflow from the vagal center is a decisive factor in WRS-induced gastric injury. The mucosal lesions were prevented by prior injection of GHRP-6 via IP did, but not via ICV, suggesting that the protection is peripheral, although a sudden injection is not equivalent to a physiological release and uptake, which eventually may affect the vagal center. CONCLUSION: From the central nervous system, vagal nerves carry the cutaneous stimuli brought about by the immersion restraint, an experimental model for inducing acute gastric erosions. GHRP-6 prevents the occurrence of these lesions.收起

学科

【SCI学科】GASTROENTEROLOGY & HEPATOLOGY 【ESI学科】CLINICAL MEDICINE

影响因子

2016年:3.365;2015年:2.787;2014年:2.369;2013年:2.433;2012年:2.547

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