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作者

Li N Wang Y Li R Liu J Zhang J Cai Y Liu S Chai T Wei L.

作者单位

Liu, Sidang Wei, Liangmeng] Sino German Cooperat Res Ctr Zoonosis Anim Origin, Tai An, Shandong, Peoples R China. [Li, Ning Liu, Jiyuan Chai, Tongjie Zhang, Jinzhou Wei, Liangmeng] Taishan Med Coll, Collaborat Innovat Ctr Origin & Control Emergng I, Tai An 271000, Shandong, Peoples R China. Wang, Yao Wei, Liangmeng] Shandong Agr Univ, Coll Anim Sci & Vet Med, Tai An 271000, Shandong, Peoples R China. [Li, Ning Cai, Yumei [Li, Ning Li, Rong

刊名

Front Microbiol

年份

2015

卷号

Vol.6

页码

425

ISSN

1664-302X

关键词

DTMUV antiviral proteins duck host innate immune response proinflammatory cytokines

摘要

Duck Tembusu virus (DTMUV) can cause serious disease in ducks, characterized by reduced egg production. Although the virus has been isolated and detection methods developed, the host immune responses to DTMUV infection are unclear. Therefore, we systematically examined the expression of immune-related genes and the viral distribution in DTMUV-infected ducks, using quantitative real-time PCR. Our results show that DTMUV replicates quickly in many tissues early in infection, with the highest viral...更多

Duck Tembusu virus (DTMUV) can cause serious disease in ducks, characterized by reduced egg production. Although the virus has been isolated and detection methods developed, the host immune responses to DTMUV infection are unclear. Therefore, we systematically examined the expression of immune-related genes and the viral distribution in DTMUV-infected ducks, using quantitative real-time PCR. Our results show that DTMUV replicates quickly in many tissues early in infection, with the highest viral titers in the spleen 1 day after infection. Rig-1, Mda5, and Tlr3 are involved in the host immune response to DTMUV, and the expression of proinflammatory cytokines (Il-1β, -2, -6, Cxcl8) and antiviral proteins (Mx, Oas, etc.) are also upregulated early in infection. The expression of Il-6 increased most significantly in the tissues tested. The upregulation of Mhc-I was observed in the brain and spleen, but the expression of Mhc-II was upregulated in the brain and downregulated in the spleen. The expression of the interferons was also upregulated to different degrees in the spleen but that of the brain was various. Our study suggests that DTMUV replicates rapidly in various tissues and that the host immune responses are activated early in infection. However, the overexpression of cytokines may damage the host. These results extend our understanding of the immune responses of ducks to DTMUV infection, and provide insight into the pathogenesis of DTMUV attributable to host factors.收起

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