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作者

Xia Liu Xingcheng Zhang Juan Li Hong Zhou Michael J Carr Weijia Xing Zhenjie Zhang Weifeng Shi

作者单位

, Ireland. Key Laboratory of Etiology and Epidemiology of Emerging Infectious Diseases in Universities of Shandong , Taishan Medical College , Taian , Shandong 271000 , People's Republic of China. School of Public Health , Taishan Medical College , Taian , Shandong 271000 , People's Republic of China. Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE) , Hokkaido University , Sapporo 001-0020 , Japan. National Virus Reference Laboratory, School of Medicine , University College Dublin , Belfield , Dublin

刊名

Journal of natural products

年份

2019

ISSN

0163-3864;1520-6025

摘要

Coxsackievirus A16 is one of the most prevalent enteroviral pathogens associated with hand, foot, and mouth disease. In the present study, we have investigated whether the bioactive compound acetylshikonin inhibits CVA16 infection in vitro and in vivo and the potential antiviral mechanism. The results suggest that AS is nontoxic at concentrations of up to 5 μmol/L and could directly inactivate virus particles at relatively low concentrations , thereby rendering CVA16 incapable of cellular en...更多

Coxsackievirus A16 is one of the most prevalent enteroviral pathogens associated with hand, foot, and mouth disease. In the present study, we have investigated whether the bioactive compound acetylshikonin inhibits CVA16 infection in vitro and in vivo and the potential antiviral mechanism. The results suggest that AS is nontoxic at concentrations of up to 5 μmol/L and could directly inactivate virus particles at relatively low concentrations , thereby rendering CVA16 incapable of cellular entry. Correspondingly, the expression of viral RNA in vitro was also reduced 100-fold when compared to infected, untreated controls. Results from a CVA16-infected neonatal mouse model indicate that, in comparison to the virus-infected, untreated group, body weights of the mice in the virus-infected, compound-treated group increased more steadily with less severe clinical symptoms. In addition, viral loads in internal organs significantly decreased in treated animals, concomitantly with both reduced pathology and diminished expression of the proinflammatory cytokines IFN-γ and IL-6. In conclusion, AS exerted an inhibitory effect on CVA16 infection in vitro and in vivo. Our study provides a basis for further investigations of AS-type compounds to develop therapeutics to mitigate CVA-associated disease in children.收起

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