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作者

Chao Huang Qiaozhen Wei Qiushi Hu Tian Wen Lei Xue Shuang Li Xiaoyan Zeng Fengjuan Shi Yongjun Jiao Lei Zhou

作者单位

Lei Zhou, National Key Laboratory of Biochemical Engineering, PLA Key Laboratory of Biopharmaceutical Production & Institute of Pathogenic Microbiology, Jiangsu Provincial Center for Disease Prevention and Control, Nanjing, China Yongjun Jiao, Institute of Pathogenic Microbiology, Jiangsu Provincial Center for Disease Prevention and Control, Nanjing 210009, China. Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China Formulation Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China School of Chemistry and Pharmaceutical Engineering, Taishan Medical University, Tai’an, China National Key Laboratory of Biochemical Engineering, PLA Key Laboratory of Biopharmaceutical Production & School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China Formulation Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.

刊名

Luminescence

年份

2019

卷号

Vol.34 No.2

页码

162-167

ISSN

1522-7243

关键词

rapid detection severe fever with thrombocytopenia syndrome virus up‐converting phosphor technology‐based lateral‐flow assay

摘要

In this study, an up‐converting phosphor technology‐based lateral‐flow assay was developed to detect severe fever with thrombocytopenia syndrome virus total antibodies rapidly and specifically. SFTSV recombinant N protein was coated on analytical membrane for sample capture, up‐converting phosphor particles were used as the reporter, the luminescence emitted by UCP particles was converted to a measurable signal by a biosensor. The performance of UPT‐LF assay was evaluated by testing 302 fiel...更多

In this study, an up‐converting phosphor technology‐based lateral‐flow assay was developed to detect severe fever with thrombocytopenia syndrome virus total antibodies rapidly and specifically. SFTSV recombinant N protein was coated on analytical membrane for sample capture, up‐converting phosphor particles were used as the reporter, the luminescence emitted by UCP particles was converted to a measurable signal by a biosensor. The performance of UPT‐LF assay was evaluated by testing 302 field serum samples by ELISA , Western blotting and UPT‐LF assay. UPT‐LF assay exhibited a lower detection limit than ELISA, and a satisfied level of agreement was exhibited by Kappa statistics . Considering Western blotting as the reference for comparison, the sensitivity and specificity of UPT‐LF assay could reach 98.31% and 100%. UPT‐LF assay showed no specific reaction with hantavirus total serum antibodies, which avoids the misdiagnosis of SFTSV from hantavirus that could cause similar clinical symptoms. UPT‐LF assay was able to achieve acceptable results within 15 min and needed only 10 μL sample for each test. As a whole, UPT‐LF assay is a candidate method for on‐site surveillance of SFTSV total antibodies owing to its excellent sensitivity, specificity, stability, easy operation and for being less time consuming.收起

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