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作者

Wang, M. Wu, Q. Xie, H. Shao, Y. Zhong, M. Zhang, X. Liu, S. He, X. Hu, S. Zhang, G.

作者单位

a Department of Hernia and Abdominal Wall Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100043, China Department of General Surgery, Qilu Hospital of Shandong University, 107#, Wenhua Xi Road, Jinan, Shandong 250012, China Department of Cardiology, Affiliated Hospital of Taishan Medical University, Taian, Shandong 271000, China

刊名

Obesity Surgery

年份

2017

卷号

Vol.27 No.11

页码

2912-2918

ISSN

0960-8923

关键词

Bariatric surgery Bile acids Liver Sleeve gastrectomy Type 2 diabetes mellitus

摘要

Background: Diabetes mellitus is a prevalent disease that endangers human health. Bariatric surgery can effectively relieve insulin resistance with elevated serum bile acids . 12α-Hydroxylated BAs were previously reported to be associated with insulin resistance. The aim of this study was to analyze changes in 12α-hydroxylated BA composition and possible associated mechanisms in diabetic rats following sleeve gastrectomy . Methods: SG and sham operations were performed in diabetic rats induced b...更多

Background: Diabetes mellitus is a prevalent disease that endangers human health. Bariatric surgery can effectively relieve insulin resistance with elevated serum bile acids . 12α-Hydroxylated BAs were previously reported to be associated with insulin resistance. The aim of this study was to analyze changes in 12α-hydroxylated BA composition and possible associated mechanisms in diabetic rats following sleeve gastrectomy . Methods: SG and sham operations were performed in diabetic rats induced by high-fat diet feeding and streptozotocin. Body weight, food intake, oral glucose tolerance test , insulin tolerance test , and serum BAs were analyzed at corresponding time points. Cholesterol 12α-hydroxylase and transcription factor V-Maf Avian Musculoaponeurotic Fibrosarcoma Oncogene Homolog G expression levels were assessed by RT-PCR and western blotting. Results: Compared with the SHAM group, the SG group displayed significant weight loss from 6 weeks postoperatively, accompanied by decreased food intake from 4 weeks after the operation. At 2 and 12 weeks postoperatively, the areas under the curve of OGTT and ITT were significantly decreased in the SG group. At 12 weeks post-operation, the SG group displayed elevated serum BAs, but the percentage of 12α-hydroxylated BAs was reduced. Furthermore, SG rats exhibited higher MAFG and lower CYP8B1 protein and mRNA levels in the liver . Conclusion: The percentage of 12α-hydroxylated bile acids was reduced after SG, which was relevant with the inhibition of CYP8B1 and overexpression of MAFG. These outcomes may play an important role in the improvement of insulin sensitivity following SG.收起

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