ZmMKK3, a novel maize group B mitogen-activated protein kinase kinase gene, mediates osmotic stress and ABA signal responses

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作者: Zhang, Maoying Pan, Jiaowen Kong, Xiangpei Zhou, Yan Liu, Yang Sun, Liping Li, Dequan

作者单位: Faculty of Chemistry and Chemical Engineering, Taishan Medical University, Tai’an 271018, Shandong, China State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an 271018, Shandong, China

关键词: 3,3′-diaminobenzidine ( DAB ) abscisic acid ( ABA ) ascorbate peroxidase ( APX ) cauliflower mosaic virus ( CaMV ) dimethylthiourea ( DMTU ) green fluorescent protein ( GFP ) Hydrogen peroxide hydrogen peroxide ( H2O2 ) methyl-viologen ( MV ) mitogen-activated protein kinase ( MAPK ) Mitogen-activated protein kinase kinase nitroblue tetrazolium ( NBT ) Osmotic stress peroxidase ( POD ) reactive oxygen species ( ROS ) superoxide dismutase ( SOD ) Transgenic tobacco

摘要: Mitogen-activated protein kinase cascades are important intracellular signaling modules and function as a convergent point for crosstalk during abiotic stress signaling. In this article, we isolated a novel group B MAPKK gene, ZmMKK3, from Zea mays. ZmMKK3 protein might be localized in both the cytoplasm and the nucleus. RNA blot analysis indicated that the ZmMKK3 transcription was up-regulated by abscisic acid , hydrogen peroxide and PEG, and that H2O2 mediated PEG-induced expression of ZmMKK...更多; Mitogen-activated protein kinase cascades are important intracellular signaling modules and function as a convergent point for crosstalk during abiotic stress signaling. In this article, we isolated a novel group B MAPKK gene, ZmMKK3, from Zea mays. ZmMKK3 protein might be localized in both the cytoplasm and the nucleus. RNA blot analysis indicated that the ZmMKK3 transcription was up-regulated by abscisic acid , hydrogen peroxide and PEG, and that H2O2 mediated PEG-induced expression of ZmMKK3. Constitutive expression of ZmMKK3 in Nicotiana tabacum reduced H2O2 accumulation under osmotic stress by affecting antioxidant defense systems and alleviated reactive oxygen species-mediated injury under oxidative stress. Transgenic tobacco exhibited attenuated ABA sensitivity by means of an increased germination rate and main root growth. Taken together, these results indicate that ZmMKK3 is a positive regulator of osmotic tolerance and ABA signaling in plants.收起

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