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作者

Famin Cao Jianhong Qi Hongqiang Song Di Xie Lu Zhou Yunning Han Hao Li Qi Wu Jun Dong Yanming Zhang

作者单位

Institute of Sports Medicine, Taishan Medical University, 619 Great Wall Road, Tai'an, 271016, Shandong, China. Institute of Sports Medicine, Taishan Medical University, 619 Great Wall Road, Tai'an, 271016, Shandong, China. jhqi7281@16 com. Institute of Sports Medicine, Taishan Medical University, 619 Changcheng Road, Tai'an, 271016, Shandong, China. jhqi7281@16 com.

刊名

Cell and tissue banking

年份

2018

卷号

Vol.19 No.4

页码

549-558

ISSN

1573-6814;1573-6814

关键词

Chondrocyte viability Culture solution Implantation Osteochondral allografts Tissue bank Vitrification

摘要

To compare the effects of Tsmu solution with vitrification on chondrocyte viability and examine histological and biomechanical properties of osteochondral allografts after storage, OCAs from femoral condyles of New Zealand rabbits were harvested, stored for 35 days in Tsmu solution or by in vitro vitrification, and subjected to in vivo and in vitro assays. Stored OCAs were transplanted into knee femoral condyle cartilage defects in recipient rabbits. Chondrocyte viability and histological chang...更多

To compare the effects of Tsmu solution with vitrification on chondrocyte viability and examine histological and biomechanical properties of osteochondral allografts after storage, OCAs from femoral condyles of New Zealand rabbits were harvested, stored for 35 days in Tsmu solution or by in vitro vitrification, and subjected to in vivo and in vitro assays. Stored OCAs were transplanted into knee femoral condyle cartilage defects in recipient rabbits. Chondrocyte viability and histological changes of cartilage grafts were assessed in vitro. Gross assessment, chondrocyte viability, histological assessment, OCA biomechanics, and immunological markers were evaluated in vivo 6 months after transplantation. Fresh OCAs served as in vitro and in vivo controls. Chondrocyte viability and scores for cartilage surface and histological quantitative assessment were superior for Tsmu solution compared with vitrification, but inferior compared with fresh OCAs in vitro and in vivo. With the exception of interleukin 6 content, biomechanical features of samples stored in Tsmu solution were superior to vitrification, and inferior to fresh OCAs in vivo. Thus, Tsmu solution provided suitable storage that improved chondrocyte viability, intact OCA cartilage matrix architecture, and transplantation outcomes.收起

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