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作者

Yan,Liu Xiao-jian,Zhu Chen,Zeng Pin-hui,Wu Hong-xiang,Wang Zhi-chao,Chen Qiu-bai,Li

作者单位

Wuhan 430022 China. ] Institute of Hematology ^' Institute of Hematology ^[10] ' Huazhong University of Science and Technology Union Hospital Taian 271000 Wuhan Central Hospital ^[12] Tongji Medical College Department of Hematology Department of Hematology ^[11] China Affiliated Hospital of Taishan Medical College

刊名

Acta pharmacologica Sinica

年份

2014

卷号

Vol.35 No.2

页码

230-238

ISSN

1671-4083

分类号

615

摘要

To investigate whether human multiple myeloma cells secrete microvesicles and whether the MVs secreted from MM cells promote angiogenesis. RPMI8226 human MM cells and EA.hy926 human umbilical vein cells were used. MVs isolated from RPMI8226 cells were characterized under laser confocal microscopy, electron microscopy and with flow cytometry. The fusion of MM-MVs and EA.hy926 cells was studied under confocal microscopy, and the transfer of CD138 to EA.hy926 cells was demonstrated with flow cyt...更多

To investigate whether human multiple myeloma cells secrete microvesicles and whether the MVs secreted from MM cells promote angiogenesis. RPMI8226 human MM cells and EA.hy926 human umbilical vein cells were used. MVs isolated from RPMI8226 cells were characterized under laser confocal microscopy, electron microscopy and with flow cytometry. The fusion of MM-MVs and EA.hy926 cells was studied under confocal microscopy, and the transfer of CD138 to EA.hy926 cells was demonstrated with flow cytometry. The proliferation, invasion and tube formation of EA.hy926 cells in vitro were evaluated using MTT, transwell migration and tube formation assays, respectively. The vasculization of EA.hy926 cells in vivo was studied using Matrigel plug assay. The expression of IL-6 and VEGF was analyzed with PCR and ELISA. MM-MVs from the RPMI 8226 cells had the characteristic cup-shape with diameter of 100-1000 nm. Most of the MM-MVs expressed phosphatidylserine and the myeloma cell marker CD138, confirming that they were derived from myeloma cells. After added to EA.hy926 cells, the MM-MVs transferred CD138 to the endothelial cells and significantly stimulated the endothelial cells to proliferate, invade, secrete IL-6 and VEGF, two key angiogenic factors of myeloma, and form tubes in vitro and in vivo. Our results confirm the presence of MVs in MM cells and support the idea that MM-MVs are newfound mediators for myeloma angiogenesis and may serve as a therapeutic target to treat MM.收起

学科

【SCI学科】CHEMISTRY, MULTIDISCIPLINARY;CHEMISTRY;PHARMACOLOGY & PHARMACY 【ESI学科】PHARMACOLOGY & TOXICOLOGY

影响因子

2018年:4.01;2017年:3.562;2016年:3.223;2015年:3.166;2014年:2.912

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期刊

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