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作者

Jianhong Qi Famin Cao Yunning Han Di Xie

作者单位

Famin Cao ( Taishan Medical University, Institute of Sports Medicine, Tai’an, China) Di Xie ( Taishan Medical University, Institute of Sports Medicine, Tai’an, China) Yunning Han ( Taishan Medical University, Institute of Sports Medicine, Tai’an, China) Jianhong Qi ( Taishan Medical University, Institute of Sports Medicine, Tai’an, China)

刊名

Cell and Tissue Banking

年份

2018

卷号

Vol.19 No.3

页码

399-404

ISSN

1389-9333;1573-6814

关键词

Osteochondral allografts Fluorescence staining Chondrocyte viability Cartilage digestion Cartilage section

摘要

The purpose of this study is to evaluate the reliability of cartilage digestion and fluorescein diacetate-ethidium bromide fluorescence staining for the detection of chondrocyte viability in osteochondral grafts. Sixteen fresh osteochondral grafts were harvested from pig knee condyles, and the articular cartilage tissue was preserved. Each cartilage graft was cut into two 70-µm thick pieces and randomly allocated to Group A or Group B. The cell viability of Group A was detected using FDA–EB flu...更多

The purpose of this study is to evaluate the reliability of cartilage digestion and fluorescein diacetate-ethidium bromide fluorescence staining for the detection of chondrocyte viability in osteochondral grafts. Sixteen fresh osteochondral grafts were harvested from pig knee condyles, and the articular cartilage tissue was preserved. Each cartilage graft was cut into two 70-µm thick pieces and randomly allocated to Group A or Group B. The cell viability of Group A was detected using FDA–EB fluorescence staining of the digested cartilage, and the viability of Group B was detected with FDA–EB fluorescence staining of cartilage sections. Comparisons of chondrocyte viability and correlation analyses of the two groups were performed using the paired sample t test and Pearson correlation test, respectively. No significant difference was found in the chondrocyte viability between Groups A and B , and a strong correlation was observed . Therefore, cartilage digestion with FDA–EB fluorescence staining is a reliable method for detecting chondrocyte viability in osteochondral grafts.收起

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