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作者单位

苏北人民医院，山东泰安 271000 泰山医学院附属医院，山东泰安 271000 西安交大扬州科技园，山东泰安 271000 泰山医学院，山东泰安 271000

刊名

Shandong Medical Journal

年份

2019

卷号

Vol.59 No.3

页码

14-17

ISSN

1002-266X

关键词

cell proliferation ability culture medium cytokine-induced killer cells cytotoxic effect effector-target ratio 培养基 效靶比 细胞因子诱导杀伤细胞 细胞增殖能力 细胞杀伤效应

摘要

Objective The efficient and stable cytokine induced killer cell culture medium and the effective target ratio of killing hepatocellular carcinoma cells were selected. Methods The peripheral blood mononuclear cells from 5 healthy people were cultured in vitro by using RPMI1640 and GT-T551 , and IL-2 was added to the two media, respectively. The morphology of the cells was observed by the optical microscope, and Trypan blue staining was used to count and draw the growth curve, and we calculated...更多

Objective The efficient and stable cytokine induced killer cell culture medium and the effective target ratio of killing hepatocellular carcinoma cells were selected. Methods The peripheral blood mononuclear cells from 5 healthy people were cultured in vitro by using RPMI1640 and GT-T551 , and IL-2 was added to the two media, respectively. The morphology of the cells was observed by the optical microscope, and Trypan blue staining was used to count and draw the growth curve, and we calculated the cell amplification multiple. CIK was cocultured with liver cancer 7721 cell line, and the effector-target ratio was adjusted to 10:1, 20:1, 40:1, and 80:1. After 72 h, we did the CCK8 test and measured OD450 by enzyme labeling instrument to calculate the killing rate. Results Compared with 1640 medium, the cell amplification multiples in the GT-T551 medium group at various concentrations of IL-2 increased . Compared with the effector-target ratio of 80:1 , the killing rate of CIK was low when the effector-target ratio was 10:1 , 20:1 and 40:1 . Conclusions The culture system of GT-T551 medium with 500 IU/mL IL-2 is more conducive to the proliferation of CIK cells. The cytotoxic effect reaches the peak when the effector-target ratio of CIK cells and 7721 cells is 80:1.收起

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