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作者

Yang, N.N. Jiao, P. Li, D.W. Wang, M.Z. Yao, S.T. Zong, C.L. Qin, S.C.

作者单位

Institute of Atherosclerosis, Taishan Medical University, Taian, 271000, China

刊名

Sheng li xue bao : [Acta physiologica Sinica]

年份

2011

卷号

Vol.63 No.6

页码

574-580

ISSN

0371-0874

关键词

EMTREE drug terms endothelial nitric oxide synthase Nos3 protein, mouse vascular endothelial growth factor A, mouse vasculotropin A EMTREE medical terms animal article bone marrow cell C57BL mouse cell culture cell differentiation cell separation culture technique cytology endothelium cell male metabolism methodology mononuclear cell mouse stem cell time MeSH Animals Bone Marrow Cells Cell Culture Techniques Cell Differentiation Cell Separation Cells, Cultured Endothelial Cells Leukocytes, Mononuclear Male Mice Mice, Inbred C57BL Nitric Oxide Synthase Type III Stem Cells Time Factors Vascular Endothelial Growth Factor A

摘要

The different biological functions were studied in mouse bone marrow-derived endothelial progenitor cells isolated by differential time attachment to obtain the optimal adherent time in this study. Density gradient centrifugation-isolated bone marrow mononuclear cells were seeded on the fibronectin-coated dish. The 1-day cultured unattached cells were seeded on the second dish for 2 more days. Then unattached cells in the second dish were seeded on the third dish. The cells on 3 dishes were defi...更多

The different biological functions were studied in mouse bone marrow-derived endothelial progenitor cells isolated by differential time attachment to obtain the optimal adherent time in this study. Density gradient centrifugation-isolated bone marrow mononuclear cells were seeded on the fibronectin-coated dish. The 1-day cultured unattached cells were seeded on the second dish for 2 more days. Then unattached cells in the second dish were seeded on the third dish. The cells on 3 dishes were defined as 1-day adherent cells, 3-day adherent cells and 3-day unattached cells, respectively. After 20-day culture, the biological functions, such as the percentage of biomarkers, the ability of adhesion, and the ability of forming tubes in vitro were analyzed. The results showed that the percentages of positive CD34, FLK-1, and CD34/FLK-1 expressions in 1-day attached cells were significantly increased compared to those in the 3-day adherent or unattached cells , which showed the strongest adhesion ability. The expression of eNOS in 1- or 3-day adherent cells was significantly higher than that in 3-day unattached cells . The expression of VEGF in 3-day adherent cells was significantly higher than that in 1-day adherent cells or 3-day unattached cells . These results suggest the biological functions of 1-day adherent cells are significantly stronger than that of 3-day adherent or unattached cells. VEGF expression in 3-day adherent cells is higher than that in 1-day adherent cells or 3-day unattached cells. The expression of eNOS in 1-day adherent cells or 3-day adherent cells is higher than that in 3-day unattached cells. The optimal adherent time to obtain mouse bone marrow-derived endothelial progenitor cells is 1-3 d.收起

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