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作者

Shuang-feng Chen Hai-ying Chen Xian-bin Liu Ying-xin Zhang Wei Liu Wei-hua Wang Bin Zhang Le-Xin Wang

作者单位

Central Laboratory for Experimental Medicine Liaocheng People's Hospital, Liaocheng and Liaocheng Clinical School of Taishan Medical University, Shandong Province, 252000 China Central Laboratory for Experimental Medicine Liaocheng People's Hospital, Liaocheng and Liaocheng Clinical School of Taishan Medical University, Shandong Province, 252000 China Department of Dental and Facial Surgery, Liaocheng People's Hospital, Liaocheng and Liaocheng Clinical School of Taishan Medical University, Shandong Province, 252000, China Central Laboratory for Experimental Medicine Liaocheng People's Hospital, Liaocheng and Liaocheng Clinical School of Taishan Medical University, Shandong Province, 252000 China Central Laboratory for Experimental Medicine Liaocheng People's Hospital, Liaocheng and Liaocheng Clinical School of Taishan Medical University, Shandong Province, 252000 China Central Laboratory for Experimental Medicine Liaocheng People's Hospital, Liaocheng and Liaocheng Clinical School of Taishan Medical University, Shandong Province, 252000 China Department of Dental and Facial Surgery, Liaocheng People's Hospital, Liaocheng and Liaocheng Clinical School of Taishan Medical University, Shandong Province, 252000, China 69332905 Corresponding author. Tel.: +61 Co-corresponding author. Tel.: +86 635 8276101 or 86 635 13346256616 School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW 2650, Australia fax: +86 635 827730 fax: +61 69 33258

刊名

Biomedicine & Pharmacotherapy

年份

2011

卷号

Vol.65 No.5

页码

322-327

ISSN

0753-3322

关键词

MG-132 Apoptosis E3 ubiquitin ligases Tca-8113 cells Squamous cell carcinoma

摘要

The aim of this study was to investigate the apoptotic effect of a proteasome inhibitor MG-132 on Tca-8113, a cell line of human tongue squamous cell carcinoma. Tca-8113 cells were treated with 10, 20, and 30 μM of MG-132, or 5 μM thapsigargin. Apoptosis rate was determined with annexin V/propidium iodide double staining. Expression of E3ubiquitin-protein ligase was determined by ELISA, and Grp78 and caspase-12 mRNA, and Grp78 and caspase-12 protein was assessed by RT-PCR and Western blot, respe...更多

The aim of this study was to investigate the apoptotic effect of a proteasome inhibitor MG-132 on Tca-8113, a cell line of human tongue squamous cell carcinoma. Tca-8113 cells were treated with 10, 20, and 30 μM of MG-132, or 5 μM thapsigargin. Apoptosis rate was determined with annexin V/propidium iodide double staining. Expression of E3ubiquitin-protein ligase was determined by ELISA, and Grp78 and caspase-12 mRNA, and Grp78 and caspase-12 protein was assessed by RT-PCR and Western blot, respectively. Apoptosis was observed 18 h after MG-132 treatment. The apoptotic rate in the 10, 20, and 30 μM MG-132 group was 13.5, 19.6 and 34.7%, respectively, which was higher than in the thapsigargin or control group . The expression of E3 ubiquitin-protein ligase in the 10, 20, and 30 μM MG-132 group was 28.75 ± 2.28, 18.16 ± 0.65, 8.85 ± 0.72, respectively, which was lower than in the thapsigargin or control group. The levels of Grp78 and capase-12 mRNA, Grp78 and caspase-12 protein in the MG-132 groups were higher than in the control group . In conclusion, MG-132 induces apoptosis in Tca-8113 cells in a concentration-dependent manner. The MG-132-induced apoptosis may involve downregulation of E3 ubiquitin ligase, and upregulation of Grp78 and caspase-12.收起

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