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作者

Lan Lin Yingying Zhang Qiongying Wei Hongguang Lin Xiaoping Li Mei-E Yu Jie Wang Zhenghui Huang Dan Xue

作者单位

School of Pharmacy, Anhui Medical University, Hefei, 230031, China Department of Respiratory Medicine, Fujian Medical University Union Hospital, Fuzhou, China Department of Geriatrics, Fujian Medical University Union Hospital, Fuzhou, China Department of Oncology, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital/Shandong Lung Cancer Institute, Shandong, China The two authors contribute equally to this work.

刊名

Talanta

年份

2022

卷号

Vol.238 Part 1

页码

123037

ISSN

0039-9140

关键词

Bidirectional activation DNA machine miRNA Synergetic effect Lung cancer

分类号

543

摘要

This work describes the unique design of a bidirectional activatable synergetic DNA machine for speeded and ultrasensitive determination of microRNA-21 , a well-known biomarker for biomedical research and early diagnosis of lung cancer. The BAS-DNA machine is composed by a pair of track strands encoding with two regions in the opposite direction for miR-21 recognition. Introduction of miR-21 can hybridize either with Track 1 or with Track 2 to activate the BAS-DNA machine with a synergistic ef...更多

This work describes the unique design of a bidirectional activatable synergetic DNA machine for speeded and ultrasensitive determination of microRNA-21 , a well-known biomarker for biomedical research and early diagnosis of lung cancer. The BAS-DNA machine is composed by a pair of track strands encoding with two regions in the opposite direction for miR-21 recognition. Introduction of miR-21 can hybridize either with Track 1 or with Track 2 to activate the BAS-DNA machine with a synergistic effect for speeded amplifying the fluorescence signal. Moreover, compared with common DNA machine with only one switch for exogenous target recognition, the BAS-DNA machine with two switches for miR-21 binding allows the speeded and strong operation of the autonomous strand scission, replication, and displacement on Track 1 and Track 2 simultaneously. This behavior makes the BAS-DNA machine powerful for ultrasensitive, specific, and fast screening of miR-21 even from real biological samples, and the fluorescence signal was found to be linear from 1 pM to 10 nM with a detection limit of 703.6 fM. We envision this BAS-DNA machine with its superior assay performance will provide a new avenue for simple, sensitive, and affordable biomedical assays.收起

学科

【SCI学科】CHEMISTRY, ANALYTICAL 【ESI学科】CHEMISTRY

影响因子

2018年:4.916;2017年:4.244;2016年:4.162;2015年:4.035;2014年:3.545

文献类型

期刊

浏览量

24