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作者

Junling Li Yan Zhang Xing Zhou Shili Wang Ruirui Hao Jinxiang Han Mian Li Yurong Zhao Cuixia Chen Hai Xu

作者单位

Qingdao West Coast New Area Marine Development Bureau, 59 Shuilingshan Road, Qingdao, 266400, China Key Lab for Biotech-Drugs of National Health Commission, 6699 Qingdao Road, Ji'nan 250117, China Biomedical Sciences College & Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences, 6699 Qingdao Road, Ji'nan 250117, Shandong, China Department of Biological and Energy Chemical Engineering, College of Chemical Engineering, China University of Petroleum (East China), 66 Changjiang West Road, Qingdao 266580, China

刊名

Journal of Colloid and Interface Science

年份

2021

ISSN

0021-9797

关键词

gelatin scaffolds RGD enzymatic functionalization host stem cell homing bone defect repair

摘要

Critical-size bone defects are imposing a substantial biomedical burden. Despite bone tissue engineering regarded as a potential approach, has virtually not proceeded to widespread clinical practices. In the BTE field, it is highly required to find a facile method to prepare active scaffolds with tailored biological functions. Here, we immobilized cell adhesive RGD motifs onto gelatin sponge scaffolds through enzymatic linking. On the basis of the resulting RGD-functionalized GS scaffolds, we...更多

Critical-size bone defects are imposing a substantial biomedical burden. Despite bone tissue engineering regarded as a potential approach, has virtually not proceeded to widespread clinical practices. In the BTE field, it is highly required to find a facile method to prepare active scaffolds with tailored biological functions. Here, we immobilized cell adhesive RGD motifs onto gelatin sponge scaffolds through enzymatic linking. On the basis of the resulting RGD-functionalized GS scaffolds, we developed a new and convenient strategy for bone defect repair, in which the scaffolds were first used to recruit mesenchymal stem cells from skeletal muscle, immediately followed by their engraftment into bone defect. We demonstrated significantly enhanced host cells homing into RGD/GS scaffolds as a result of specific RGD-integrin interactions, and the recruited host cells showed a strong osteogenic differentiation potential. After ectopic implantation of cell-laden RGD/GS scaffolds into critical-size mouse bone defects, marked bone tissue regeneration occurred. The presented strategy not only provides an agile route for the preparation of bioactive scaffolds and the construction of osteoinductive bone-graft substitutes, but also avoids or minimizes the complicated and laborious cell isolation, in vitro expansion and cell seeding procedures used in the conventional BTE.收起

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