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作者

Baibing Zheng, Xingguo Song, Li Wang, Yue Zhang, Youyong Tang, Shiwen Wang, Lei Li, Yawen Wu, Xianrang Song, Li Xie

作者单位

Affiliations ¹ Department of Clinical Laboratory, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, China. ² School of Medical Laboratory, Weifang Medical University, Weifang, China. ³ Shandong Provincial Key Laboratory of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, China.

刊名

Frontiers in oncology

年份

2022

卷号

Vol.12

页码

1037523

ISSN

2234-943X

关键词

NSCLC biomarker diagnosis exosomal tRFs plasma.

摘要

Background: tRNA derived small RNAs have recently received extensive attention; however, the effects of tRFs in exosome as biomarkers has been less studied. The objective of this study was to validate novel diagnostic exosomal tRFs with sensitivity and specificity for non-small cell lung cancer . Methods: Exosomes extracted from plasma of NSCLC patients and healthy individuals were identified by transmission electron microscopy , qNano and western blots. The differentially expressed tRFs were s...更多

Background: tRNA derived small RNAs have recently received extensive attention; however, the effects of tRFs in exosome as biomarkers has been less studied. The objective of this study was to validate novel diagnostic exosomal tRFs with sensitivity and specificity for non-small cell lung cancer . Methods: Exosomes extracted from plasma of NSCLC patients and healthy individuals were identified by transmission electron microscopy , qNano and western blots. The differentially expressed tRFs were screened by high-throughput sequencing in plasma exosomes of NSCLC patients and healthy individuals, and further verified by Quantitative Real-Time PCR . To assess the diagnostic efficacy of exosomal tRFs for NSCLC, receiver operating characteristic curves were used next. Results: The expression levels of exosomal tRF-Leu-TAA-005, tRF-Asn-GTT-010, tRF-Ala-AGC-036, tRF-Lys-CTT-049, and tRF-Trp-CCA-057 were significantly decreased in NSCLC patients and early-stage NSCLC patients compared to healthy individuals. Notably, the exepression of tRF-Leu-TAA-005, tRF-Asn-GTT-010, tRF-Ala-AGC-036, tRF-Lys-CTT-049, and tRF-Trp-CCA-057 in the exosomes were higher than the exosome depleted supernatant . Conclusions: Our results showed that the levels of exosomal tRF-Leu-TAA-005, tRF-Asn-GTT-010, tRF-Ala-AGC-036, tRF-Lys-CTT-049, and tRF-Trp-CCA-057 were significantly downregulated in NSCLC patients. This suggests that these five exosomal tRFs may be promising diagnostic biomarkers for NSCLC.收起

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