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作者

Shiyou Wang, Xiaohui Li, Chao Liu, Yi Yuan, Feng Ma

作者单位

Affiliations ¹ Key Laboratory of Synthetic Biology Regulatory Elements, Institute of Systems Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, China. ² Suzhou Institute of Systems Medicine, Suzhou, 215123, China. ³ Department of Radiation Oncology and Shandong Provincial Key Laboratory of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, 250117, China. ⁴ Research Unit of Radiation Oncology, Chinese Academy of Medical Sciences, Jinan, 250117, China. ⁵ Department of Medical Research, Jiangsu Toneker Pharmaceutical Technology Co., Ltd., Suzhou, 215123, China.

刊名

Journal of medical virology

年份

2022

ISSN

1096-9071

关键词

HPV cervical cancer infiltration single-cell RNA sequencing tumor-associated macrophage.

摘要

Almost all cases of cervical cancer can be attributed to high-risk HPVs infections in keratinocytes. However, it is unknown whether HPV invades immune cells such as macrophages and T cells. We analyzed the single-cell transcriptome of the CC and its adjacent tissues and found that HPV16 genes, including E1, E6, and E7, expressed in the macrophages and CD8 T cells in addition to the malignant cells. HPV16 macrophages highly expressed the genes that promote cell adhesion and the favorable genes s...更多

Almost all cases of cervical cancer can be attributed to high-risk HPVs infections in keratinocytes. However, it is unknown whether HPV invades immune cells such as macrophages and T cells. We analyzed the single-cell transcriptome of the CC and its adjacent tissues and found that HPV16 genes, including E1, E6, and E7, expressed in the macrophages and CD8 T cells in addition to the malignant cells. HPV16 macrophages highly expressed the genes that promote cell adhesion and the favorable genes such as WAS, IQCB1, MYO1F, and PDZD11 in CC prognosis. The transcription factor KLF5 potentially accounted for the induction of these protective genes and thus facilitated the infiltration of the immune cells in tumor tissues. Our single-cell transcriptome analysis suggests the potential value of the HPV16 macrophage in CC prognosis. However, extensive experimental studies investigating the characteristics and functions of the HPV immune cells are still required. This article is protected by copyright. All rights reserved.收起

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