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作者

Donglin Zhou Yimin Zhu Peng Jiang Tongfu Zhang Jianfeng Zhuang Tao Li Linzeng Qi Yunyan Wang

作者单位

¹Department of Neurosurgery, Qilu Hospital of Shandong University, Cheeloo College of Medicine and Institute of Brain and Brain-Inspired Science, Shandong University, 107 Wenhua Western Road, 250012, Jinan, Shandong, China ⁵Department of Neurosurgery, The Third Affiliated Hospital of Shandong First Medical University, Jinan, China ⁴Department of Neurosurgery, Yangxin County People’s Hospital, Binzhou, China ³Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy, Cancer Institute, Xuzhou Medical University, Xuzhou, China ²Department of Orthopedics, Qilu Hospital of Shandong University, Jinan, China

刊名

Biological Research

年份

2023

卷号

Vol.56 No.1

页码

50

ISSN

0717-6287

关键词

Intracranial aneurysm Pyroptosis Inflammatory cell death Gene expression omnibus Bioinformatics analysis

摘要

Background Intracranial aneurysm is the most common cerebrovascular disease, and subarachnoid hemorrhage caused by its rupture can seriously impede nerve function. Pyroptosis is an inflammatory mode of cell death whose underlying mechanisms involving the occurrence and rupture of IAs remain unclear. In this study, using bioinformatics analysis, we identified the potential pyroptosis-related genes and performed their inflammatory response mechanisms in IAs. Methods The mRNA expression matrix...更多

Background Intracranial aneurysm is the most common cerebrovascular disease, and subarachnoid hemorrhage caused by its rupture can seriously impede nerve function. Pyroptosis is an inflammatory mode of cell death whose underlying mechanisms involving the occurrence and rupture of IAs remain unclear. In this study, using bioinformatics analysis, we identified the potential pyroptosis-related genes and performed their inflammatory response mechanisms in IAs. Methods The mRNA expression matrix of the IA tissue was obtained from the Gene Expression Omnibus database, and 51 PRGs were obtained from previous articles collected from PubMed. The differentially expressed PRGs were performed using R software. Subsequently, we performed enrichment analysis, constructed a protein–protein interaction network, performed weighted gene coexpression network analysis and external validation using another dataset, and identified a correlation between hub genes and immune cell infiltration. Finally, the expression and tissue distribution of these hub genes in IA tissues were detected using Western blotting and immunohistochemical staining. Results In total, 12 DEPRGs associated with IA were identified in our analysis, which included 11 up-regulated and one down-regulated genes. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that the DEPRGs were mostly enriched in the NOD-like receptor signaling pathway, interleukin-1 beta production, and the inflammasome complex. Three hub genes, NLRP3 , IL1B and IL18 , were identified using Cytoscape software and the WGCNA correlation module, and external validation revealed statistically significant differences between the expression of these hub genes in the ruptured and unruptured aneurysm groups . Conclusion The results of bioinformatics analysis showed that pyroptosis is closely related to the formation and rupture of IA, and identified three potential hub genes involved in the pyroptosis and infiltration ofcells. Our findings may improve the understanding of the mechanisms underlying pyroptosis in IA.收起

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