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作者

Xinqiu Xu Chaofan Jia Fengjiao Zhang Hao Li Weilei Gong Changqin Wang & …Yin Wei

作者单位

⁴School of Public Health, Binzhou Medical University, Yantai, China ¹School of Public Health, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, China ²School of Public Health, Jining Medical University, Jining, China ³Key Laboratory of Food Nutrition and Safety, School of Public Health, Jining Medical College, Jining, China

刊名

Journal of Fluorescence

关键词

Vitamin D3 Aptamer Fullerenes Fluorescence

摘要

This study presents a rapid, simple aptamer-based fluorescence sensor using fullerenes as a quencher for detecting vitamin D3. Fullerenes, with its π-electron cloud, acts as a powerful electron acceptor, facilitating Förster resonance energy transfer from carboxyfluorescein . The aptamer, labeled with 5′6-FAM, adsorbs onto the fullerenes surface through hydrogen bonding and π-π stacking interactions, leading to fluorescence quenching. Upon vitamin D3 binding, the aptamer forms a hairpin structu...更多

This study presents a rapid, simple aptamer-based fluorescence sensor using fullerenes as a quencher for detecting vitamin D3. Fullerenes, with its π-electron cloud, acts as a powerful electron acceptor, facilitating Förster resonance energy transfer from carboxyfluorescein . The aptamer, labeled with 5′6-FAM, adsorbs onto the fullerenes surface through hydrogen bonding and π-π stacking interactions, leading to fluorescence quenching. Upon vitamin D3 binding, the aptamer forms a hairpin structure that prevents adsorption onto the fullerenes surface, restoring fluorescence. The fullerenes were characterized using UV–Vis, FT-IR, Raman spectroscopy, SEM, TEM, and zeta potential measurements. The sensor's response to varying vitamin D3 concentrations was analyzed with a fluorescence spectrometer, revealing a linear detection range of 0–600 nM and a detection limit of 200 nM, which improved to 50 nM with exonuclease I. The sensor demonstrated a recovery rate of 88.4%–96.3% for vitamin D3 in water samples, confirming its feasibility for practical applications. In addition, our proposed sensor exhibited good repeatability and stability . The innovative use of fullerenes as a fluorescence quencher, along with the aptamer's specific binding to vitamin D3, provides a novel and highly sensitive avenue for vitamin D3 detection.收起

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