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作者

Li Zhang, Keyu Liu, Xiuyun Duan, Shan Zhou, Hailin Jia, Yingnan You, Bo Han

作者单位

com. ¹⁰ Medical Science and Technology Innovation Center, Shandong First Medical University, Shandong Academy of Medical Sciences, Jinan, Shandong, China. hanbo35@16 com. ⁸ Shandong Provincial Clinical Research Center for Children's Health and Disease office, Shandong Provincial Hospital, Jinan, Shandong, China. hanbo35@16 com. ⁹ Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong, China. hanbo35@16 com. ⁷ Department of Pediatric Cardiology, Cheeloo College of Medicine, Shandong Provincial Hospital, Shandong University, Jinan, Shandong, China. hanbo35@16 Affiliations ¹ Department of Pediatric Cardiology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. ² Shandong Provincial Clinical Research Center for Children's Health and Disease office, Shandong Provincial Hospital, Jinan, Shandong, China. ³ Medical Science and Technology Innovation Center, Shandong First Medical University, Shandong Academy of Medical Sciences, Jinan, Shandong, China. ⁴ Department of Pediatric Cardiology, Cheeloo College of Medicine, Shandong Provincial Hospital, Shandong University, Jinan, Shandong, China. ⁵ Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong, China. ⁶ Department of Pediatric Cardiology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. hanbo35@16 com.

刊名

Journal of translational medicine

年份

2025

卷号

Vol.23 No.1

页码

399

ISSN

1479-5876

关键词

CD8+T cell CXCR4 Myocarditis Single-cell RNA sequencing.

摘要

Background: Myocarditis is a common inflammatory heart disease in children and young adults, with fulminant myocarditis being the most severe form due to its rapid onset and high mortality rate. However, the precise pathological immune subsets and molecular change in myocarditis, particularly FM, remain unknown. Methods: We performed single-cell RNA sequencing of pediatric peripheral blood mononuclear cells during the acute and recovery phases of FM. A viral myocarditis mouse model was establi...更多

Background: Myocarditis is a common inflammatory heart disease in children and young adults, with fulminant myocarditis being the most severe form due to its rapid onset and high mortality rate. However, the precise pathological immune subsets and molecular change in myocarditis, particularly FM, remain unknown. Methods: We performed single-cell RNA sequencing of pediatric peripheral blood mononuclear cells during the acute and recovery phases of FM. A viral myocarditis mouse model was established using CVB3. Deletion and adoptive transfer of CD8T cells, as well as blockade of CXCR4, were conducted in vivo. CD8T cells were sorted and cultivated in vitro, then stimulated with CXCL12 and CXCR4 antagonists to investigate the mechanism of CD8T cell overactivation. Results: CD8T cells show significant activation, amplification, enhanced cytotoxicity, and increased chemotactic ability in FM. Deletion of CD8T cells alleviates myocardial injury and improves cardiac function in MC mice, while adoptive transfer of CD8T cells from MC mice aggravates myocardial inflammation and injury. The transcriptomic analysis reveals elevated CXCR4 expression in CD8T cells in acute FM. In vitro experiments demonstrate that the CXCL12/CXCR4 axis drives the overactivation and cytotoxicity of CD8T cells. In vivo treatment with a CXCR4 antagonist effectively reduces CD8T cell accumulation in the heart, alleviates myocardial inflammation, and improves cardiac function in MC mice. Conclusions: These findings provide deeper insights into the immune landscape of pediatric FM, uncovering a novel role of the CXCL12/CXCR4 axis in driving CD8T cell responses in myocarditis. Furthermore, they highlight the CXCL12/CXCR4 axis as a promising therapeutic target for myocarditis treatment.收起

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