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作者

Chuansong Quan, Lu Wang, Jiming Gao, Yaoni Li, Xiaoyu Xu, Houqiang Li, Zixuan Gao, Wenxu Ruan, Hongzhi Liu, Qian Li, Weijia Xing, Liqiong Zhao, Michael J Carr, Weifeng Shi, Haifeng Hou

作者单位

Affiliations ¹ Key Laboratory of Emerging Infectious Diseases in Universities of Shandong, Shandong First Medical University and Shandong Academy of Medical Sciences, Ji'nan, China. ² The Second Affiliated Hospital of Shandong First Medical University, Taian, China. ³ School of Public Health, Shandong First Medical University and Shandong Academy of Medical Sciences, Ji'nan, China. ⁴ Medical Records and Statistics Management Office, Tengzhou Central People's Hospital, Zaozhuang, China. ⁵ Department of Neurobiology and Physiology, School of Clinical and Basic Medicine, Shandong First Medical University and Shandong Academy of Medical Sciences, Ji'nan, China. ⁶ Baoji Central Hospital, Baoji, China. ⁷ School of Life Sciences, Shandong First Medical University and Shandong Academy of Medical Sciences, Taian, China. ⁸ National Virus Reference Laboratory, School of Medicine, University College Dublin, Dublin, Ireland. ⁹ International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Japan. ¹⁰ Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. ¹¹ Shanghai Institute of Virology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

刊名

eLife

年份

2025

卷号

Vol.14

页码

RP106989

ISSN

2050-084X

关键词

B cell glycosylation haemorrhagic fever with renal syndrome hantaan virus immunoglobulin G immunology infectious disease inflammation microbiology viruses.

摘要

Haemorrhagic fever with renal syndrome is a fatal disease caused by Hantaan virus infection. Humoral immunity is essential for effective viral clearance; however, the glycosylation characteristics of immunoglobulin G in HFRS patients are not well known. Peripheral blood mononuclear cells from HFRS patients were obtained for B subset analysis using scRNA-seq and flow cytometry. HTNV-specific IgG antibody titers were detected by enzyme-linked immunosorbent assay, and IgG glycosylation was analy...更多

Haemorrhagic fever with renal syndrome is a fatal disease caused by Hantaan virus infection. Humoral immunity is essential for effective viral clearance; however, the glycosylation characteristics of immunoglobulin G in HFRS patients are not well known. Peripheral blood mononuclear cells from HFRS patients were obtained for B subset analysis using scRNA-seq and flow cytometry. HTNV-specific IgG antibody titers were detected by enzyme-linked immunosorbent assay, and IgG glycosylation was analyzed by ultra-performance liquid chromatography. The proportions of the antibody-secreting memory B cells and plasmablasts were significantly expanded among acute HFRS patients. We discovered significantly increased fucosylated IgG and decreased bisecting N-acetylglucosamine during the convalescent phase of HTNV infection. Meanwhile, positive correlations were observed between ASM subsets and galactosylation/sialylation in the IgG Fc region, and between PB subsets and sialylation. Notably, the glycosylation-related genes, such as RPN1 and RPN2, were primarily expressed differentially in the ASM and PB subclusters, which were enriched in the N-glycosylation modifications of proteins through asparagine. Our findings indicated that IgG N-glycosylation may play a crucial role in combating HTNV infection and contributing to clinical recovery, which provided new insights for optimizing glycoengineered therapeutic antibodies.收起

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