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作者

Guo, Shoudong Mao, Wenjun Yan, Mengxia Zhao, Chunqi Li, Na Shan, Jimiao Lin, Cong Liu, Xue Guo, Tao Guo, Tiantian Wang, Shuyao

作者单位

Key Laboratory of Atherosclerosis in Universities of Shandong Province Yushan Road, Qingdao 266003, People’s Republic of China Institute of Atherosclerosis, Taishan Medical University, Taian, Shandong 271000, People’s Republic of China a Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China,

刊名

Carbohydr Polym

年份

2014

卷号

Vol.105

页码

325-333

关键词

Aspergillus ochraceus ES-CID MS/MS Extracellular polysaccharide Galacto-oligosaccharide NMR

摘要

The homogeneous extracellular polysaccharide, AW1, was obtained from the fermented broth of the fungus Aspergillus ochraceus derived from coral Dichotella gemmacea. AW1 was a galactomannan with a molar ratio of mannose and galactose of 2.16:1.00 and a molecular weight of about 29.0kDa. The structure of AW1 was investigated by chemical and spectroscopic methods, including methylation analysis, one- and two-dimensional nuclear magnetic resonance (1D, 2D NMR) and electrospray mass spectrometry with...更多

The homogeneous extracellular polysaccharide, AW1, was obtained from the fermented broth of the fungus Aspergillus ochraceus derived from coral Dichotella gemmacea. AW1 was a galactomannan with a molar ratio of mannose and galactose of 2.16:1.00 and a molecular weight of about 29.0kDa. The structure of AW1 was investigated by chemical and spectroscopic methods, including methylation analysis, one- and two-dimensional nuclear magnetic resonance (1D, 2D NMR) and electrospray mass spectrometry with collision-induced dissociation (ES-CID MS/MS) spectroscopic analyses. The results showed that the backbone of AW1 consisted of (1⟶2)-linked α-d-mannopyranose residues. The mannopyranose residues in the backbone were substituted at C-6 by the (1⟶)-linked α-d-mannopyranose units and (1⟶5)-linked β-d-galactofuranose oligosaccharides with different degrees of polymerization. The investigation demonstrated that AW1 was a novel galactomannan with different structural characteristics from other fungal galactomannans, and could be a potential resource of the (1⟶5)-linked β-d-galactofuranose oligosaccharides.收起

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