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作者

Wang, Z.-D. Qu, F.-Y. Chen, Y.-Y. Ran, Z.-S. Liu, H.-Y. Zhang, H.-D.

作者单位

Interventional Radiology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, 264100, China Department of Basic Medicine, Taishan Medical University, Tai’an, 271001, China Center of Health Examination, Affiliated Hospital of Taishan Medical University, Tai’an, 271001, China a Clinical Laboratory of Taishan Sanatorium of Shandong Province, Tai’an, 271001, China Department of Oncology, Affiliated Hospital of Taishan Medical University, Tai’an, 271001, China

刊名

Journal of Zhejiang University: Science B

年份

2017

卷号

Vol.18 No.1

页码

27-36

ISSN

1673-1581

关键词

Early growth response protein 3 (EGR3) Hepatocellular carcinoma (HCC) Malignant phenotype MicroRNA miR-718

摘要

Objective: Hepatocellular carcinoma is still one of the most common death-related malignancies worldwide. Because the way onset and progression are hidden most, HCC diagnoses are made at an advanced stage, when they are unsuitable for surgical resection. MicroRNAs are a class of small non-coding RNAs, participating in many aspects of cancers. In this study, we tried to establish the role of microRNA-718 in the malignant phenotype of HCC cells and its possible role in HCC diagnosis. Methods: He...更多

Objective: Hepatocellular carcinoma is still one of the most common death-related malignancies worldwide. Because the way onset and progression are hidden most, HCC diagnoses are made at an advanced stage, when they are unsuitable for surgical resection. MicroRNAs are a class of small non-coding RNAs, participating in many aspects of cancers. In this study, we tried to establish the role of microRNA-718 in the malignant phenotype of HCC cells and its possible role in HCC diagnosis. Methods: Here we first used a methylthiazolyldiphenyl-tetrazolium bromide assay, Transwell migration and invasion assays, and colony formation assay to evaluate the impact of miR-718 on the malignant phenotypes of HCC cells. Then, we used bioinformatic methods to predict the target gene of miR-718 and used green fluorescence protein reporter assay, Western blot, and quantitative real-time polymerase chain reaction to validate the regulation relationship. Finally, we determined the role of the target gene in the HCC phenotype. Results: We found that the expression of miR-718 was significantly reduced in various HCC cell lines and HCC tissues. Re-expression of miR-718 significantly reduced the cellular viability and colony formation ability as well as inhibited the migration and invasion abilities of HCC cell lines. Early growth response protein 3 is a direct target of miR-718 and is negatively regulated by miR-718. EGR3 could increase the viability and proliferation of HCC cells, and promot the migration and invasion of HCC cells. Conclusions: miR-718 acts as a tumor suppressive microRNA in HCC via regulating the expression of EGR3, which may provide a new diagnostic marker and treatment target for HCC.收起

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