Expression, purification and crystallization of Chaetomium thermophilum Cu,Zn superoxide dismutase

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作者: Wakadkar, S., Zhang, L.-Q., Li, D.-C.Email Author, Haikarainen, T., Dhavala, P., Papageorgiou, A.C. View Correspondence

作者单位: Department of Chemistry and Chemical Engineering, Taishan Medical College, Taian, Shandong 271016, China Department of Environmental Biology, Shandong Agricultural University, Taian, Shandong 271018, China a Turku Centre for Biotechnology, University of Turku and Åbo Akademi, Turku 20521, Finland

刊名: Acta Crystallographica Section F: Structural Biology and Crystallization Communications

摘要: Cu,Zn superoxide dismutase from the thermophilic fungus Chaetomium thermophilum was expressed in Pichia pastoris and purified. Crystals were grown in over 120 conditions but only those produced with 1.4 M sodium potassium phosphate pH 8.2 as precipitant were suitable for structural studies. Data were collected to 1.9 Å resolution at 100 K from a single crystal using a synchrotron-radiation source. The crystals belonged to space group P61/P65, with unit-cell parameters a = 90.2, c = 314.5 Å and ...更多; Cu,Zn superoxide dismutase from the thermophilic fungus Chaetomium thermophilum was expressed in Pichia pastoris and purified. Crystals were grown in over 120 conditions but only those produced with 1.4 M sodium potassium phosphate pH 8.2 as precipitant were suitable for structural studies. Data were collected to 1.9 Å resolution at 100 K from a single crystal using a synchrotron-radiation source. The crystals belonged to space group P61/P65, with unit-cell parameters a = 90.2, c = 314.5 Å and eight molecules in the asymmetric unit. Elucidation of the crystal structure will provide insights into the active site of the enzyme and a better understanding of the structure-activity relationship, assembly and thermal stability of Cu,ZnSODs.收起

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