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作者

Kun Fang, Da-Lang Fang, Hui Yu, Yu-Ang Chen, Pei-Ze Yu, Zi-Fan Wang, Rui-Bin Zhang, Wen Yang, Lei Tao, Hiroshi Fukushima, Yang Dong, Cong-Hui Han

作者单位

Affiliations ¹ Nanjing University of Chinese Medicine, Nanjing, China. ² The Third Affiliated Hospital of Shandong First Medical University, Affiliated Hospital of Shandong Academy of Medical Sciences, Jinan, China. ³ Department of Gland Surgery, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China. ⁴ Department of Urology, Yantai Hospital of Traditional Chinese Medicine, Yantai, China. ⁵ Department of Urology, Xuzhou Clinical School of Xuzhou Medical University, Xuzhou, China. ⁶ Department of Nephrology, Central Hospital Affiliated to Shandong First Medical University, Jinan, China. ⁷ Department of Urology, Tokyo Medical and Dental University, Tokyo, Japan. ⁸ Department of Urology, Xuzhou Central Hospital Affiliated to Nanjing University of Chinese Medicine, Xuzhou, China. ⁹ Medical College of Soochow University, Suzhou, China.

刊名

Translational andrology and urology

年份

2024

卷号

Vol.13 No.5

页码

812-827

ISSN

2223-4691

关键词

Bladder cancer (BC) microRNA sequencing (miRNA-seq) microRNAs (miRNAs) solasonine.

摘要

Background: Solasonine has been demonstrated to exert an inhibitory effect on bladder cancer , but the potential mechanisms remain unclear. Therefore, the aim of this study is to explore the association between microRNAs -mediated regulation and the anti-tumor activities of solasonine in BC. Methods: MiRNA sequencing was performed to identify the differentially expressed microRNAs associated with solasonine in BC cells. Functional enrichment analyses of the DE-miRNAs activated and inhibited by ...更多

Background: Solasonine has been demonstrated to exert an inhibitory effect on bladder cancer , but the potential mechanisms remain unclear. Therefore, the aim of this study is to explore the association between microRNAs -mediated regulation and the anti-tumor activities of solasonine in BC. Methods: MiRNA sequencing was performed to identify the differentially expressed microRNAs associated with solasonine in BC cells. Functional enrichment analyses of the DE-miRNAs activated and inhibited by solasonine were then conducted. The DE-miRNAs with prognostic value for BC and those differentially expressed in the BC samples were subsequently identified as the hub DE-miRNAs. After identifying the messenger RNAs that were targeted by the hub DE-miRNAs and those differentially expressed in the BC samples, a protein-protein interaction analysis was performed to identify the core downstream genes, which were then used to construct a solasonine-miRNA-mRNA regulatory network. Results: A total of 27 activated and 19 inhibited solasonine-mediated DE-miRNAs were identified that were found to be associated with several tumor-related biological functions and pathways. After integrating the results of the survival analysis and expression assessment, the following nine hub DE-miRNAs were identified: hsa-miR-127-3p, hsa-miR-450b-5p, hsa-miR-99a-5p, hsa-miR-197-3p, hsa-miR-423-3p, hsa-miR-4326, hsa-miR-625-3p, hsa-miR-625-5p, and hsa-miR-92a-3p. The DE-mRNAs targeted by the hub DE-miRNAs were predicted, and 30 core downstream genes were used to construct the solasonine-miRNA-mRNA regulatory network. miR-450b-5p was shown to be associated with the most mRNAs in this network, which suggests that it plays a crucial role in the solasonine-mediated anti-BC effect. Conclusions: A regulatory network, including solasonine, miRNAs, and mRNAs related to BC, was constructed. This network provides extensive insights into the molecular regulatory mechanisms that underlie the anti-cancer efficacy of solasonine in BC.收起

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