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作者

Kun Fang Da-Lang Fang Hui Yu Yu-Ang Chen Pei-Ze Yu Zi-Fan Wang Rui-Bin Zhang Wen Yang Lei Tao Hiroshi Fukushima Yang Dong Cong-Hui Han

作者单位

Nanjing University of Chinese Medicine，Nanjing，China；The Third Affiliated Hospital of Shandong First Medical University，Affiliated Hospital of Shandong Academy of Medical Sciences，Jinan，China；Department of Gland Surgery，Affiliated Hospital of Youjiang Medical University for Nationalities，Baise，China；Department of Urology，Yantai Hospital of Traditional Chinese Medicine，Yantai，China；Department of Urology，Xuzhou Clinical School of Xuzhou Medical University，Xuzhou，China；Department of Nephrology，Central Hospital Affiliated to Shandong First Medical University，Jinan，China；Department of Urology，Xuzhou Clinical School of Xuzhou Medical University，Xuzhou，China；Department of Nephrology，Central Hospital Affiliated to Shandong First Medical University，Jinan，China；The Third Affiliated Hospital of Shandong First Medical University，Affiliated Hospital of Shandong Academy of Medical Sciences，Jinan，China；The Third Affiliated Hospital of Shandong First Medical University，Affiliated Hospital of Shandong Academy of Medical Sciences，Jinan，China；Department of Urology，Tokyo Medical and Dental University，Tokyo，Japan；Department of Urology，Xuzhou Clinical School of Xuzhou Medical University，Xuzhou，China；Department of Urology，Xuzhou Central Hospital Affiliated to Nanjing University of Chinese Medicine，Xuzhou，China；Medical College of Soochow University，Suzhou，China；Nanjing University of Chinese Medicine，Nanjing，China；Department of Urology，Xuzhou Clinical School of Xuzhou Medical University，Xuzhou，China；Department of Urology，Xuzhou Central Hospital Affiliated to Nanjing University of Chinese Medicine，Xuzhou，China；Medical College of Soochow University，Suzhou，China

刊名

泌尿男科转化医学杂志

年份

2024

卷号

第13卷

期号

第5期

页码

812-827

关键词

Bladder cancer (BC) solasonine microRNAs (miRNAs) microRNA sequencing (miRNA-seq)

摘要

BackgroundSolasonine has been demonstrated to exert an inhibitory effect on bladder cancer , but the potential mechanisms remain unclear. Therefore, the aim of this study is to explore the association between microRNAs -mediated regulation and the anti-tumor activities of solasonine in BC.MethodsMiRNA sequencing was performed to identify the differentially expressed microRNAs associated with solasonine in BC cells. Functional enrichment analyses of the DE-miRNAs activated and inhibited by solas...更多

BackgroundSolasonine has been demonstrated to exert an inhibitory effect on bladder cancer , but the potential mechanisms remain unclear. Therefore, the aim of this study is to explore the association between microRNAs -mediated regulation and the anti-tumor activities of solasonine in BC.MethodsMiRNA sequencing was performed to identify the differentially expressed microRNAs associated with solasonine in BC cells. Functional enrichment analyses of the DE-miRNAs activated and inhibited by solasonine were then conducted. The DE-miRNAs with prognostic value for BC and those differentially expressed in the BC samples were subsequently identified as the hub DE-miRNAs. After identifying the messenger RNAs that were targeted by the hub DE-miRNAs and those differentially expressed in the BC samples, a protein-protein interaction analysis was performed to identify the core downstream genes, which were then used to construct a solasonine-miRNA-mRNA regulatory network.ResultsA total of 27 activated and 19 inhibited solasonine-mediated DE-miRNAs were identified that were found to be associated with several tumor-related biological functions and pathways. After integrating the results of the survival analysis and expression assessment, the following nine hub DE-miRNAs were identified: hsa-miR-127-3p, hsa-miR-450b-5p, hsa-miR-99a-5p, hsa-miR-197-3p, hsa-miR-423-3p, hsa-miR-4326, hsa-miR-625-3p, hsa-miR-625-5p, and hsa-miR-92a-3p. The DE-mRNAs targeted by the hub DE-miRNAs were predicted, and 30 core downstream genes were used to construct the solasonine-miRNA-mRNA regulatory network. miR-450b-5p was shown to be associated with the most mRNAs in this network, which suggests that it plays a crucial role in the solasonine-mediated anti-BC effect.ConclusionsA regulatory network, including solasonine, miRNAs, and mRNAs related to BC, was constructed. This network provides extensive insights into the molecular regulatory mechanisms that underlie the anti-cancer efficacy of solasonine in BC.收起

基金

国家自然科学基金;江苏省自然科学基金

文献类型

期刊